1' Prepare roughened gold electrodes, and take SERS'

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1. Prepare roughened gold electrodes, and take
SERS. 2. Stabilize gold prism by phosphane, and
take electrophoresis. 3. Stabilize gold prism
with C11. 4. Made RSA gold prism monolayer.
Yan, Wei group, 05/28/04
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Prepare roughened gold electrode Gold foils were
treated with propene torch and sonicated for
10min. Electrochemistry treatment for 30
sweeps Solution 0.1M KCl EI-300mV DI1.3s Scan50
0mV/s EI1.2V DI30s SERS was taken by NIRM(
784.45nm) , ?40 objective, 10s
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SERS of freshly prepared roughened gold
electrode. There is always a peak at 566nm.
After dried, this peak disappeared. After soaked
in CS2, a peak at 472nm appeared, which was lowed
when soaked in THF.
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Roughened gold electrode was dried, soaked in
CS2, dried, and soaked in different concentration
of Didecylamine THF solution, successively.
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Roughened gold electrode was dried, soaked in
CS2, dried, and soaked in different concentration
of Morpholine THF solution successively.
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Roughened gold electrode was dried, soaked in
CS2, dried, and soaked in different concentration
of Piperidine THF solution successively.
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• Stabilize gold prism particles
• 10mL Au prism particle sol(40nm) was treated with
  MB-3 for overnight.
• added with 4mg dipotassium bis(p-sulfonatophenyl)
  phenylphosphane dihydrate for 10 hours.
• Centrifugated 2 at 5000RPM for 10min, remove the
  supernatant and redispersed the particles in 5ml
  water.
• Beomseok told me to try C11 first. I can not form
  layer by C11, and the particles tend to aggregate.

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Electrophoresis 2 agarose, TBE buffer, voltage
97V The particles with different shape in the
gold prism sol seemed to be separated. The gel
was cut according to different color, and
dissolved in a kind of salt solution. Due to the
small volume, it is difficult to get the sample
after centrifugation.
1 hour later 2
hours later Others are 5nm gold particle treated
with different DNA segments.
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As the gold prism can be stabilized by phosphane
this time, we can made a monolayer on PEI coated
glass slide. As the density is still low, the
SERS is not good.
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• Conclusions
• Compared with the former results, the SERS of
  morpholine in THF is similar to pure morpholine,
  but different to morpholine in water.
• the SERS of piperidine in THF is similar to pure
  piperidine, but different to piperidine in water.
  
• the SERS of didecylamine in THF is similar to
  didecylamine in water.
• When soaked in different concentration of
  solution successively, the increase in SERS is
  low. While soaked in different concentration of
  solution respectively, the increased in SERS is
  significant.
• Next week
• Make roughened gold electrodes, and take SERS by
  soaking the electrodes in different concentration
  of THF solution respectively.
• Separate particles with different shape from gold
  prism sol by electrophoresis.
• Treated the gold prism with Beomseoks molecule.