Selfrenewal

1
What is Stem Cells?
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- Self-renewal - Multi-potent
Differentiation
2
Source of Stem Cells
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Embryonic versus Adult Stem Cells
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Embryonic Stem Cells
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Bone marrow (BM) stem cellsCord blood (cord)
stem cellsAdipose-derived stem
cellsTissue-specific stem cells
Plasticity of Adult Stem Cells
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Stem Cells in the Bone Marrow
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Colony Formation of Adult BM Cells

• (40X)

CFU-F colony forming unit-fibroblast BFU-E
burst forming unit-erythroid CFU-G colony
forming unit-granulocyte CFU-M colony forming
unit- macrophage CFU-pre-B colony forming
unit-B-lymphoid progenitor CFU-GM colony
forming unit-granulocyte macrophage CFU-GEMM
colony forming unit-granulocyte erythroid
macrophage megakaryocyte
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Differentiation of HSCs
Hematopoietic Stem Cells
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The Mesengenic Process
Proliferation
Bone marrow periosteum
Commitment
Differentiation
Mesenchymal Tissue
Maturation
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Mesenchymal Stem Cells (MSCs)

• General Characteristics

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Isolation Culture of MSCs
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Differentiation of MSCs (I)
Myogenesis
Adipogenesis
Control
Control
Desmin
Differentiated
Differentiated
Troponin
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Differentiation of MSCs (II)
Chondrogenesis
Osteogenesis
Alkaline phosphatase
Col II control
GAG - control
Bone nodules
Col II TGF-b
GAG TGF-b
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Cell Surface Molecular Profile of hMSCs
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Surface Marker Analysis of hMSCs (I)
MSCs markers
HSCs markers
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Surface Marker Analysis of hMSCs (II)
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Marker Gene Expression of hMSCs during
Chondrogenic Differentiation
Chondrogenesis
Dedifferentiation