Design of Enzyme Reactor

1
Design of Enzyme Reactor

• Geoff Greer
• Joseph Gvildys
• Aaron McGregor
• April 2nd, 2007

2
Enzyme Selection

• Transpeptidation
• Trypsin or Achromobacter lyticus proteinase I
• Optimal enzyme conditions
• 4oC and a pH of 7

Eli Lilly
3
Enzyme Immobilization

• Benefits
• Savings on enzyme requirements and downstream
  processing
• Trypsin-Agarose
• 211 for 100 units ( 3mL)

Property
Biological Source trypsin from bovine pancreas
Extent of Labeling 15-35 units per ml (packed gel)
Matrix beaded agarose
Storage Temperature 2-8oC
4
Reactor Comparison

• Batch
• High operating costs
• Batch-to-batch variations
• PFR
• Difficult to control pH and Temperature
• CSTR
• Simple pH, Temperature control
• Simple catalyst charging and replacement

5
Design Parameters
Parameter Value
Q 7.786 L/h
Ro 0.0179 mol/L
Vmax 2.35 10-7 mol/L.s
Km 9.90 10-5 mol/L
k2 0.1285 s-1
MWreactant 6000 g/mol
MWtrypsin 25 000 g/mol
6
Reactor Design

• Specify conversion, calculate R
• From R, calculate v
• From v, calculate D

7
Reactor Design

• From D, calculate V
• From V, calculate tres
• From Vmax, k2, calculate Eo and total amount of
  enzyme required

8
Reactor Sizing
9
Reactor Sizing

• Conversion of 99.25
• 120 L reactor required
• Increase conversion by 0.5 to 99.75
• 360 L reactor required

10
Enzyme required

• Required amount of enzyme is
• 1.83 µmol/L
• 5.5 g pure trypsin
• 2.6 kg immobilized trypsin
• Based on difference in activity between
  immobilized and non-immobilized enzyme
• 18 000

11
Reactor Design Considerations

• Temperature control
• pH control
• Level control
• Sterilizable
• Wire-mesh sieve
• Sample port

12
Simplified Reactor Schematic
13
Costing of the Reactor

• Several different manufacturers
• New Brunswick Scientific
• Novaferm AB
• Scigenics
• New MBR
• Quote from New Brunswick Scientific
• BioFlo Pro 150 L (120 L working volume)
• 140 000 (US)
• Batch reactor

14
Final Reactor Schematic
15
Overall Cost of Unit

• For reactor and enzyme, estimated cost is at
  least 160 000
• Additional considerations
• Reactor modifications
• De-naturation of enzyme

16
Thank You

• Questions?