Stem Cell Technology in Regenerative Medicine and Cancer Treatment

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Stem Cell Technology in Regenerative Medicine and
Cancer Treatment

• Gary Van Zant, Ph.D.
• University of Kentucky
• Markey Cancer Center

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Differentiation of HumanTissues
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Derivation of embryonic stem cell lines
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Advantages and limitations of current methods for
deriving pluripotent stem cells
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Figure 1 Derivation of pluripotent stem cells
Müller R and Lengerke C (2009) Patient-specific
pluripotent stem cells promises and challenges
Nat Rev Endocrinol doi10.1038/nrendo.2009.18
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Embryonic Stem Cells
An Immortal Pluripotential Resource
Immortal continually replicating
Bulk Culture
ES cells
ES Cell Bank
Academic Research Institutions Globally
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Somatic Nuclear Transfer (SMNT)
ES cells
Compatible transplants
Nuclear transfer
Two cell types needed
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Somatic cells eg, skin fibroblasts
Source of eggs self, mother, relative, egg bank
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Derivation of pluripotent stem cells
Induced pluripotent stem cells (iPCs)
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Potential for Tissue Regeneration Using Stem Cells
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Adult Stem Cells
?
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Although controversial, there is some evidence of
stem cell plasticity
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Interactions between stem cells and their
supporting microenvironment are important