Title: THE CHARACTERIZATION OF 4 TIBETAN ARTEMIA STRAINS AND THE HUFA PROFILE DURING THE ENRICHMENT AND STA
1THE CHARACTERIZATION OF 4 TIBETAN ARTEMIA
STRAINS AND THE HUFA PROFILE DURING THE
ENRICHMENT AND STARVATION
- Xiuling Yu, Naihong Xin, Juju SunSalt Research
Institute
2Characterization of 4 Artemia strains
- 1. Biometrics
- 2. Hatching rate
- 3. Fatty acid profile
3 4 Tibetan Salt Lakes
- 1. Gaize lake (GZ)
- 2. Shuanghu Lake (SH)
- 3. Nima Lake (NM)
- 4.Yangnapengco Lake (YP).
4Table 1. Location, elevation and surface area of
4 Tibetan lakes
- Lake Location Elevation (m)
Area (km2) - GZ 84?05?E32?02?N 4490 86
- YP / /
12 - SH 91?10?E31?13?N 4664 140
- NM 86?07?E30?28?N 4663 25
5Table 2 Biometrics of Artemia from 4 Tibetan
lakes
6Table 3 Hatching rate of Artemia cysts from 4
Tibetan lakes at 28 ?C (hrs)
7Table 4 HUFA content of decapsulated cysts from
4 Tibetan lakes (mg/g,D.W.)
8Enrichment and Starvation
- Two Artemia strains 1. Great Salt Lake(EG)
- 2.Laguoco Lake
(AT) - Three enrichment diets 1. DC Selco
- 2. DC
Selco - 3. A1 Selco
9Conditions
- Enrichment800ml 35ppt seawater, 27?C,
- density 300/ml(EG), 200/ml(AT),
- 0.6g/l (two ration)
- Starvation 800ml 35ppt seawater, 12 ?C,
- Density 900ml/(EG),600/ml(AT),
- The samples were taken at 6 hrs,12 hrs and 24hrs
10Table 5 The HUFA content (mg/g, D.W) of
A.franciscana (EG) and A.tibetiana after 24
hours enrichment and during starvation at 12 ?C.
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12Note24E after 24 hours enrichment,
6h/12 ?C store the enriched nauplii at 12?C
for 6 hours, 12h/12 ?C store the
enriched nauplii at 12 ?C for12
hours, 24h/12 ?C store the enriched nauplii at
12 ?C for 24 hours
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15The metabolism of A.franciscana(EG) and
A.tibetiana(AT) nauplii enriched with Super Selco
during cold storage at 12?C
16The metabolism of A.franciscana(EG) and
A.tibetiana(AT) nauplii enriched with A1 Selco
during cold storage at 12?C
17The decrease of DHA/EPA ratio of
A.franciscana(EG) and A.tibetiana(AT) nauplii
enriched with Super Selco during the cold storage
at 12?C
18The decrease of DHA/EPA ratio of
A.franciscana(EG) and A.tibetiana(AT) nauplii
enriched A1Selco during the cold stored at 12?C