Growing cells in culture

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BIOE 202 Lab 2
Jan 29
Growing cells in culture
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Online Quiz
Review
2 the volume of trypan blue must be exact PBS
for rinsing, TRED for lifting, and media for
resuspending do not 3 cell lines are immortal
due to a genetic mutation
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Online Quiz
Review
4 this culture is 95 confluent 5 this
culture is 50 confluent
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Online Quiz
Review
6 7 8
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Online Quiz
Review
9 unused cells should be bleached for at least 5
min, then poured down the sink with plenty of
water 10 TRED trypsin EDTA, it is used to
detach cells from the culture dish 11. Contact
inhibition means that cells halt growth when they
touch each other
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Prelab Exercise
Answer
For a T-25 flask with 18 and 30 hour doubling
times