Bez nadpisu

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GENE-MINE WP-E4 Task E4-2
Acquisition of Lactuca serriola seed samples
Task leader PU Participants RICP, IPK, HRI,
PRI Duration month 8-9 2001
Objectives Acquisition of suitable seed samples
for research purposes from 50 different
populations from the European
distribution sites of L. serriola
Czech Republic, Germany - 16 samples United
Kingdom - 10 samples The Netherlands - 8 samples
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Aims of the Task E4-2
following the collecting protocol the required
number of populations representing distribution
and ecogeographic variation of the country was
collected each collector should have provided
1) seed samples 2) collecting protocols for each
collecting site 3) photodocumentation of habitat
and L. serriola plants
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The Czech Republic - collection site data of L.
serriola

• Collecting 13. - 29. August 2001
• 16 seed samples collected from 16 localities all
  16 samples f. serriola
• Population size 20-320 plants Population area
  25-1250 m2
• Geographical characteristics 4906'59"-5033'32"N
  , 1414'10"-1732'46"W, altitudes 177-405 m
• Exposures south (8 loc.), all round (4 loc.)
• Landforms from lowland (8 loc.) to hilly (8
  loc.)
• Soil textures loamy (5 loc.), loamy-sandy (4
  loc.), stony (5 loc.), anthropogenic
• Site habitats roadsides (3 loc.), field margins
  (4 loc.), dumps of building material (4 loc.)
• Dominant plant species L. serriola, Matricaria
  maritima, Arrhenantherum elatius, Urtica dioica,
  Chenopodium album, Artemisia vulgaris
• Phenology flowering and fruiting
• Diseases only one locality without any diseases,
  E. cichoracearum (10 loc.) infection degree 1,
  Bremia lactucae (15 loc.) infection degree 1

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The Czech Republic - Lactuca serriola collection
sites
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Germany - collection site data of L. serriola

• Collecting 15. August - 5. October 2001
• 16 seed samples collected from 16 localities 14
  samples f. serriola, 2 samples bulk of both forms
  (f. serriola f. integrifolia)
• Population size 15-400 plants Population area
  20-12000 m2
• Geographical characteristics 4740'42"-5404'19"N
  , 618'34"-1321'12"E, altitudes 15-410 m
• Exposures south (5 loc.), south-west (3 loc.)
• Landforms valley (2 loc.), plain (13 loc.),
  hilly (1 loc.)
• Soil textures clays (6 loc.), loams (5 loc.),
  sandy (4 loc.)
• Site habitats roadsides (12 loc.)
• Dominant plant species Urtica dioica, Arthemisia
  sp., Daucus carota, Plantago sp.
• Phenology flowering and fruiting
• Diseases E. cichoracearum (7 loc.)

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Germany -Lactuca serriola collection sites
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The Netherlands - collection site data of L.
serriola

• Collecting 22. August - 9. September 2001, 3
  samples collected in 1998
• 8 seed samples collected from 8 localities 3
  samples f. integrifolia, 2 samples f. serriola, 8
  samples bulk of both forms
• Population size 30-300 plants Population area
  50-500 m2
• Geographical characteristics 5050'42"-5229'19"N
  , 0353'38"-0606'59"E, altitudes -1-50 m
• Exposures not documented
• Landforms not documented
• Soil textures prevalence of sandy soils (5
  loc.), clays (2 loc.)
• Site habitats ruderals, building sites (6 loc.)
• Dominant plant species L. serriola, Erigeron
  canadensis, Matricaria maritima, Chenopodium
  album
• Phenology prevalence of seed set
• Diseases E. cichoracearum on 4 localities,
  Bremia lactucae not recorded

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The Netherlands - Lactuca serriola collection
sites
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The United Kingdom - collection site data of L.
serriola

• Collecting 23. August - 9. September 2001
• 10 seed samples collected from 10 localities
  forms of L. serriola were not documented
• Population size 20-1000 plants Population area
  6-10000 m2
• Geographical characteristics 5122'12"-5242'00"N
  , 146'34"W-008'52"E, altitudes -11-96 m
• Exposures mostly all round
• Landforms plain (4 loc.), hilly (2 loc.)
• Soil textures loams (5 loc.), clays (3 loc.)
• Site habitats field margins (4 loc.), ruderals
  (4 loc.)
• Dominant plant species not documented
• Phenology flowering, prevalence of seed set
• Diseases mildew - on 1 locality (not specified),
  Diptera larvae on 4 localities

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The United Kingdom - Lactuca serriola collection
sites
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Conclusions

• The number of planned seed samples was collected.
  
• The quality of most seed samples was quite good,
  however after cleaning the amount of some samples
  (from the Netherlands and UK) was rather low
  (only ca 20 seeds). This could be considered as a
  critical amount for obtaining sufficient number
  of vital plants, however regeneration yielded
  sufficient amount of seeds for experimental
  purposes.
• The quality of collecting data. High quality of
  data was received from Germany and the Czech
  Republic. In some aspects (e.g. forms of L.
  serriola, exposures, landforms, diseases) the
  data originating from the Netherlands and UK were
  rather poor (in both countries the form of L.
  serriola was not determined).
• Photodocumentation is available only from Czech
  Republic.
• Complete set of data is important for complex
  consideration and interpretation of results
  (collecting data are available on diskette).

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L. serriola habitats in the Czech Republic