Peroxidase Lab

1
Peroxidase Lab

• Question What are the signs that a chemical
  change has taken place?
• Materials Chicken liver, Hydrogen peroxide,
  Erylenmeyer flask, stopper to fit flasks, wood
  splint, matches

2
Procedure

• Add 40 mL hydrogen peroxide to the flask
• Stopper the flask
• Record Observations

3
Procedure continued

• Remove stopper from flask
• Insert a wooden splint into the neck of the flask
• Record observations

4
Procedure continued

• Light a match and ignite the wood splint, blow
  out the flame and insert the splint into the neck
  of the flask
• Record Observations

5
Procedure continued

• Add the hydrogen peroxide to the flask.
• Stopper the Flask
• Record observations

6
Procedure continued

• Leave liver in closed flask for 2 min.
• Light the splint and blow out the flame
• Insert into the flask.
• Record your observations
• Repeat number 13 and 14 but also stopper the
  flask
• Record your observations

7
Questions- Regular

• Why did you make observations after each step of
  the procedure?
• What observations did you make that indicate a
  chemical change occurred?
• Why is it important for the stopper to be
  replaced quickly after it has been removed?
• Do you think that other materials could be
  substituted in this experiment that would give
  you the same results? Why or why not?

8
Questions- Enhanced

• Why did you make observations after each step of
  the procedure?
• What observations did you make that indicate a
  chemical change occurred?
• Why is it important for the stopper to be
  replaced quickly after it has been removed?
• Do you think that other materials could be
  substituted in this experiment that would give
  you the same results? Why or why not?
• If you could redesign the lab, how would you
  change it? Explain why you would use these
  changes.