What is a Gene

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What is a Gene?

• Comprised of a coding region
• -encodes information for proteins mRNA
• -other RNA
• and regulatory regions
• -noncoding sequences 5 and 3 to coding region
• -proteins bind to these sequences

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Prokaryotic Gene Structure
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Pribnow Box
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(No Transcript)
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Translation of Multiple Genes from a Single mRNA
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Eukaryotic Gene Structure
3
5
TATA
CAAT
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Genomic DNA has Exons Introns mRNA Doesnt
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Eukaryotic mRNA Synthesis Requires Transcription
and Processing
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Processing the Ovalbumin Gene Transcript
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DNA to mRNA Hybridization
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RNA Splicing
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DNA Transcription Unit Features
Promoter elements Core promoter elements
TATA box
Initiator (Inr) Downstream
promoter element (DPE) Transcription
factor (TF) binding sites Response Elements
CAAT boxes GC boxes
SP-1 sites GAGA
boxes Enhancer Repressor site(s)
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Examples of Core Promoters
TATA TATA box
Inr Initiator
DPE Downstream Promoter Element