RFLP Restriction Fragment Length Polymorphism

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RFLPRestriction Fragment Length Polymorphism

• Marie Cerná,
• Markéta Cimburová,
• Marianna Romžová

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RFLP Restriction Fragment Length Polymorphism

• restriction analysis of DNA
• by its digestion
• with restriction endonucleases (RE)
• in specific restriction sites

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RFLP Restriction Fragment Length Polymorphism

• in the case the sequence difference
• (polymorphism) creates or disturbs
• a specific site for RE,
• after restriction, fragments with
• different sizes are formed

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Principle

• starting DNA
• (genomic DNA, PCR product)
• digestion with a restriction enzyme into
  fragments with different sizes
• fragments electrophoresis separation

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Restriction endonucleases (RE)

• known about 2100 bacterial RE
• RE recognize variously short nucleotides
  sequences (4,6,8),
• in which then they digest covalent
  phosphodiester bonds

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???

• Do RE often digest, which recognize shorter or
  longer sequences?
• What is a role of RE in natural systems?