Use of the Labnet Micropipettor

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Use of the Labnet Micropipettor
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Purpose of the Pipettor

• Biotechnologists use a pipettor to transfer
  precise amounts of liquids from one container to
  another.
• The measurements are in microliters 1 ml1000 µl.

Use of the Labnet Micropipettor
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Care of the Pipettor

• Misuse of the pipettor can result in incorrect
  measurements affecting your investigation.
• Do not drop the pipettor.
• Do not adjust the volume to less than 2 µl.

Use of the Labnet Micropipettor
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Parts of the Pipettor
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• 1) yellow pushbutton
• 2) calibration knob
• 3) digital volume display
• 4) barrel
• 5) disposable pipette tip
• 6) tip ejector

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Use of the Labnet Micropipettor
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Volume AdjustmentStep 1

• In the display window are three vertical numbers.
• The top number is tens µl (10.0).
• The middle number is ones µl (02.0).
• The bottom number tenths µl (00.9).

Use of the Labnet Micropipettor
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Volume AdjustmentStep 2

• Set the pipettor to the desired volume by turning
  the calibration knob until the volume display
  reads the desired numbers.
• 7.5 µl would read from top to bottom in the
  display window 0
  - tens 7
  - ones .5 - tenths

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Transferring LiquidsStep 1

• Adjust the pipettor to the desired volume.
• Hold the pipettor so the display is facing the
  user and the thumb can easily depress the yellow
  pushbutton.

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Transferring LiquidsStep 2

• Open the box of yellow pipette tips supplied by
  the Office of Biotechnology.
• Place the pipette cone (end) into one of the tips
  and press down firmly.
• NEVER USE THE PIPETTOR WITHOUT A TIP!

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Transferring LiquidsStep 3

• Lift the pipettor and attached tip away from the
  tip box.
• Depress the pushbutton to the first stop before
  placing the pipette tip in the liquid.
• Place the tip in the liquid.

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Transferring LiquidsStep 4

• Slowly release the pushbutton and draw the liquid
  into the tip.
• Remove the tip from the liquid and visually check
  for liquid in the tip.

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Transferring LiquidsStep 5

• Place the tip into the container which is to
  receive the liquid.
• Slowly depress the pushbutton as far as it can
  go.
• With the pushbutton fully depressed, withdraw the
  tip.

Use of the Labnet Micropipettor
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Transferring LiquidsStep 6

• Press down on the tip ejector button to eject the
  tip.
• Dispose of the tip in an appropriate container.

Use of the Labnet Micropipettor