Nomenclature

1
Nomenclature
2
Reactions

• Substrates (reactants) -- A B C D
• Products -- P Q R S
• Enzyme -- E F G
• Inhibitors -- I J
• Activators
• Metal ion -- Mn/-
• Other -- X Y Z

3
Enzymes

• Stable when isolated from the rest of the
  reaction components, it has a long half-life with
  respect to the assay times scale, and reactants
  add to it in a bimolecular step.
• Transitory when isolated from the rest of the
  reaction components its half-life is short
  relative to the assay time scale.
• Central if the active site is completely filled
  and kinetically competent
• Noncentral if not.

4
Kinetic Mechanism

• Sequential all reactants must be bound to an
  enzyme before any reaction occurs.
• Ping-Pong a product is released between the
  addition of two substrates
• Ordered there is an obligatory order fo addition
  of reactants to an enzyme and /or release of
  products from enzyme.
• Random there is not.

5
Kinetic Constants

• Michaelis constant (Km) is defined as the
  concentration of reactant that gives half the
  maximum rate at saturating concentrations of all
  other reactants and with products maintained at
  zero concentration.

6
Rate Constants

• Microscopic associated rate constants of the
  individual steps in a reaction scheme
• First-order
• Second-order
• Macroscopic

7
(No Transcript)
8
(No Transcript)
9
(No Transcript)
10
(No Transcript)
11
(No Transcript)
12
Michaelis-Menten