BCell Generation,

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Chapter 11 B-Cell Generation,
Activation, and Differentiation
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???? 1. B-Cell Maturation 2. B-Cell Activation
and Proliferation 3. The Humoral Response 4. In
Vivo Studies for Induction of Humoral Response 5.
Germinal Centers and Antigen-Induced B-Cell
Differentiation 6. Regulation of B-Cell
Development 7. Regulation of the Immune Effector
Response
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B-Cell Maturation
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Overview of B-cell Development Sites of
B-cell maturation before birth yolk sac
fetal liver
fetal bone marrow after birth
bone marrow
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Bone-Marrow Stromal Cells Are Required for
Maturation of Progenitor B Cells into Precursor B
Cells
Direct contact is required
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Sequence of Events and Characteristics of the
Stages in B Cell Maturation in the Bone Marrow
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• Pre-B Cell Receptor
• Heavy chain m Chain
• Surrogate light chain VpreB and l5
• Iga/Igb

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Sequential Expression of Membrane Ig and
Surrogate Light Chain at Different Stages of
B-Cell Differentiation in the Bone Marrow
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Experimental Evidence for Negative Selection of
Self- Reactive B Cells During Maturation in the
Bone Marrow
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B-Cell Activation and Proliferation
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Two Distinct Signals for B-Cell Activation
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The Initial Stages of Signal Transduction by an
Activated B-Cell Receptor (BCR)
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Signal Transduction Pathways Activated by the BCR
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BCR Complex TAPA-1 (CD81), CD2 (CD21), and CD19
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Sequence of Events in B-Cell Activation by a
Thymus-Dependent Ag
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Initial Contact between a T Cell and B Cell
a T-B Conjugate
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Clonal Anergy in Mature Peripheral B Cells
Production of double-transgenic mice carrying
transgenes encoding HEL and anti-HEL Ab
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FACS Analysis of Peripheral B Cells that Bind
HEL Compared with Membrane IgM Level
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Clonal Deletion of Self-Reactive Mature
Peripheral B Cells
Production of double-transgenic mice expressing
the class I Kb molecule and anti-Kb Ab
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FACS analysis of bone marrow and peripheral
(lymph node) B cells for Kb binding versus mIgM
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Concentration and Isotype of Serum Ab Following
Primary (1 ) and Secondary (2 ) Immunization
with Ag
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Hapten-Primed and Carrier-Primed Cells Are
Separate Populations
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A Peripheral Lymph Node
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Cellular Events within Secondary Follicles of
Peripheral Lymph Nodes
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The Frequency of Somatic Hypermutation Decreases
with the Distance from the Rearranged V(D)J Gene
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Interactions of Numerous Cytokines with B Cells
Generate Signals Required for proliferation and
Class Switching During the Differentiation of B
Cells into Plasma Cells
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The End
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Clonal Anergy in Mature Peripheral B Cells