www'tcellbiology'orgteaching'htm

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Simon Davis, Ed Evans ?21336
www.t-cellbiology.org/teaching.htm
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The expression strategy
Secreted or membrane bound?
Your protein

• 3. Mammalian cells
• (e.g. CHO K1 cells)
• moderately fast
• very high yields potentially (lt400 mg/l)
• sugars can be removed
• (Lec3.2.8.1 cells)
• transient or stable

needs to be glycosylated or dont want to
refold?
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