Genetic Memory

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Genetic Memory Culture Memory
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Genetic Memory
The code is the same for all life forms on earth
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Culture Memory

• Recent 10,000 years
• Transmission by writing, book, image, parents,
  school, social group,
• Scientific Knowledge exponential rising during
  the last 3 centuries.

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What is life ? (E. Schrödinger, 1944)
Life is DNA
( Water Proteins lipids carbohydrates)
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What is DNA ?
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The Human Genome
Genes ( introns promoters) 3 Retroelements
retroviruses 8 retrotransposons 13 LTR 23 To
tal 47 Repeats 50 LINE 22 SINE
13 Unknown
?
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Epigenetic DNA Circulating Voyager DNA 
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Mass Pyrosequencing and Assembly Technology
Detect Unique Serum DNA Profiles in Treatment
Naïve Breast Cancer Patients
Howard B. Urnovitz, Julia Beck and Ekkehard
Schütz   Chronix Biomedical GmbH, Goetheallee 8,
37075 Goettingen, Germany
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454 Life Sciences/Roche Mass Sequencing
The DNA sequence
A droplet with one cellular message
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Serum DNA and Cancer

• Serum DNA Studies to Date
• Prion Disease Proof of Concept
• Serum DNA and Breast Cancer
• Summary

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TOTAL NUCLEOTIDE INVENTORY
Human Germline DNA

• Circulating DNA
• Traces from Damaged Cells
• Detects disease related events

• Gut Flora/Viral DNA
• 1013-1014 microorganisms
• 100 x more genes than humans

• Mitochondrial DNA
• Circular
• 37 genes
• 16 kb
• 2-10 copies per MT

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Healthy Human CNA Profile
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Two Applications of MPSA Approach
Routine Lab Tests for

• Screening for Specific Cancers
• Small number of target sequences that define a
  cancer type to screen population
• Personalized Monitoring
• Following more target sequences (obtained from
  patient tumor cells) to monitor an individual

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Summary

• Mass Sequencing and Assembly Technology Reveals
  CNA Order
• Significant difference between genomic DNA and
  CNAs are revealed in SINES (mostly Alus) and
  LINEs (most significantly with L2)
• Comparing Treatment Naïve Breast Cancer CNAs to
  the apparently healthy database, thousands of
  discrepancies are revealed
• Bioinformatic search engines show that a subset
  of transposable elements (n lt10 categories) can
  define breast cancer samples in ROC curve
  analyses with a C-Statistic gt 0.9.
• The unique sequences represent lt0.2 of CNAs

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Another promising approach DNA from pathogenic
bacteria and viruses detected in blood plasma by
electromagnetic signals
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Capture of the signals
Signal Analysis software
X 500
Sample
Computer
Sensor coil
Amplifier
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Plasma from patient with Alzheimer Disease
NF
F D-2
Dilution 10-2 negative
F D-8 (-)
F D-9 ()
F D-3
F D-4
F D-10 ()
F D-19 (-)
Dilution 10-8 positive
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• Results bacterial DNA detected in patients with
  
• Chronic Lyme disease
• Rhumatoïd arthritis
• Alzheimer disease
• Parkinson disease
• Multiple sclerosis
• Some neuropathies
• Some forms of autism
• Cancers

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Neurodegenerative diseases
Carbohydrates Proteins
Mutations Expression of retroelements
Infectious agents
Oxidative Stress
DNA
Cancer
lipids
Immune System Dysfunctions