INVESTIGATION%20OF%20MONO-ISOPROPYLNAPHTHALENE,%20DI-ISOPROPYLNAPHTHALENE%20AND

1
INVESTIGATION OF MONO-ISOPROPYLNAPHTHALENE,
DI-ISOPROPYLNAPHTHALENE AND TRI-ISOPROPYLNAPHTHALE
NE IN THE ENVIRONMENT AROUND THE PAPER RECYCLING
PLANT
Suzuki M, Matsumura C, Moriguchi Y, Nakano
T Hyogo Prefectural Institute of Public Health
and Environmental Sciences, 3-1-27 Yukihira-cho,
Suma-ku, Kobe, Hyogo, 654-0037 Japan
Introduction
Sampling
Mono-Isopropylnaphthalene ( MIPN ),
Di-Isopropylnaphthalene ( DIPN ) and
Tri-Isopropylnaphthalene ( TIPN ) are widely used
for PCBs substitutes such as jet printer ink,
pesticide solvent and so on.
Air sampling at Point C
Point A
Ibo River
Point B
They are concerned to have harmful influences to
health and environment because of their high
persistent and accumulating properties, but their
fate in the environment had not been
illustrated. Their optimum analytical methods for
environmental samples had not been developed.
Point D
Point C
The Paper Recycling Plant
MIPN
DIPN
Point D
TIPN
(1,3-DIPN)
(1,3,5-TIPN)
(1-MIPN)
Point E
purpose
Wastewater from Paper Recycling plant
1. Development of their simultaneous analytical
methods for the water, the air, the sediment and
the biological samples. 2. Investigation of
their concentration and isomer fingerprints in
the environmental samples around the paper
recycling plant.
Point F
Hyogo Pref.
Ibo river at Point F
GC/MS Condition
Off the coast of Himeji
GC/MS Agilent6890N/JMS-Q1000GC
Point G
ColumnSupelcowax-10(Polyethleneglycol)
(0.32mm30m0.25µm)
MS
GC
Interface240?
Carrier GasHe(1mL/min)
Results
Ion Source210?
Injection
Split less(Purge ON 1.5min)
Ionization300µA, 70eV
Injection Volume 2µL
Detection ModeSIM
Injection Temp. 270?
Monitor Ion
254?212?170?156?142
DIPN
TIPN
Oven50?(1)?20?/min?100??5? /min?210??20?/min?270?
(1)
HCB-13C6 ( Internal Standard )
290, 255
Sediment
Algae
Sediment
Algae
Water
N.A.
N.A.
N.A.
N.D.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
N.A.
Fig.2 Separated DIPN isomer peaks by columns.
Fig.1 The chromatogram of MIPN, DIPN and TIPN
standard.
N.A.
N.A.
N.D.
DIPN isomers peaks was rather separated by using
polar column than medium polar one.
N.A.
Sea bass
MIPN and DIPN isomers can be assigned and 6 TIPN
isomers peaks was separated.
N.A.
Air
News paper
Analytical procedure
Water sample
Shaking or mixing
(the roof of our institute)
Concentrated to 200µL
Fig.3 The concentration of DIPN and TIPN in
samples.
1L water sample
Clean up by sulfuric acid
Extracted by 10mL Hexane
Analyzed by GC/MS
NaCl
30g
Sediment and Biological sample
Concentrated to 200µL
Analyzed by GC/MS
20g sample
methanol-hexane partition
Cleaned up
Extracted by alkaline degradation with 50mL
KOH/MeOH for about 12 hours.
Fig.4 The fingerprints of DIPN isomers in
samples. Y axis indicates a ratio to 2,6-DIPN.
by the silica gel containing 5 water or florisil
or sulfuric acid
N.D.
Air sample
Conclusion
1. The simultaneous analytical methods of MIPN,
DIPN and TIPN in the water, the air, the sediment
and the biological samples were developed.
Concentrated to 200µL
Analyzed by GC/MS
2. DIPN and TIPN were detected from the water,
the sediment, the air and the biological samples
and MIPN was not detected all samples.
Sampling
0.5 m3 10m3 air were introduced into PS-Air
Extracted by 30mL DCM
3. The wastewater and the exhaust from the paper
recycling plant were one of loading sources of
DIPN.
Recovery
4. It was considered that there was another
pollution resource of DIPN and TIPN in the Ibo
River because high concentration of TIPN and the
unusual fingerprint of DIPN isomers were detected
from the sediment which was not influenced by the
wastewater from the recycled paper factory.
5. It was considered that DIPN in sea bass was
metabolized because the rates of decomposition
were different by isomers.
amount20ng or 40ng
Detection limit (water)
References
Unit ng/L
Brzozowski R, Skupinski W, Jamroz H, M,
Skarzynski M, Otwinowska H. Journal of
Chromatography A 2002946