DNA Fingerprinting Gel Staining and Analysis Student Instructions

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DNA FingerprintingGel Staining and
AnalysisStudent Instructions
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Gel Staining and AnalysisStep 1

• When electrophoresis is completed, turn off the
  power supply.
• Remove the lid from the box.
• Lift the lid to remove it from the box.

DNA Fingerprinting Student Instructions
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Gel Staining and AnalysisStep 2

• To stain the gel to make the DNA band visible,
  remove the gel tray from the electrophoresis unit
  and place the tray into a plastic container.
• Slide the gel off the tray by pushing on one end
  of it.

DNA Fingerprinting Student Instructions
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Gel Staining and AnalysisStep 3

• Add the Carolina Blu DNA Stain, making sure the
  gel is completely immersed. Do not pour the stain
  directly on the gel.
• Stain the gel for 15 minutes. Agitate gently, if
  possible.

DNA Fingerprinting Student Instructions
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Gel Staining and AnalysisStep 4

• Pour the stain back into the bottle.
• Cover the gel with distilled water to destain.
• Agitate gently, if possible, for 30 to 40
  minutes, changing the water every 10 minutes.

DNA Fingerprinting Student Instructions
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Gel Staining and AnalysisStep 5

• The gel can be displayed to the class by sliding
  it onto a piece of plexiglass and placing it on a
  white sheet of paper or on a white-light viewing
  box.

DNA Fingerprinting Student Instructions
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Gel Staining and AnalysisStep 6

• Ideal gel
• Stained with ethidium bromide and viewed under UV
  light source

DNA Fingerprinting Student Instructions