PCR

1
PCR The Polymerase Chain ReactionTheory and
Applications
A three-day short course
Dave Palmer, Bio-Rad dpalmer_at_zdap.com http//zdap.
com/pcr
2
April 14, 2007
AM

• PCR History
• GMO Investigator Kit Background
• Preparation of GMO food samples
• How PCR Works
• GMO PCR Reaction Preparation

PM
3
April 21, 2007
AM

• Recap
• Pipetting technique and Electrophoresis
• Set up GMO PCR agarose gels
• Hair follicle DNA collection
• GMO PCR results analysis
• PCR Primer Design
• Crime Scene PCR kit overview
• Set up Crime Scene PCR reactions

PM
4
April 28, 2007
AM

• Recap
• Pour CSI and D1S80 gels
• Advanced PCR and Troubleshooting
• Load CSI and D1S80 gels
• Analyze and interpret gels
• Real-Time PCR lecture
• Set up real-time PCR reactions
• Final questions?
• Final Exam

PM
5
Course Goals

• Introduction to PCR
• Basic PCR methods
• PCR troubleshooting
• Agarose gel electrophoresis
• Understanding of real-time PCR
• Basic PCR lab skills
• Pipetting technique
• Basic DNA extraction
• Agarose gel electrophoresis
• Experimental design and use of controls

6
Course Evaluation

• Open-book test, short answer, 30min
• Three practical uses of PCR
• Basic components of a PCR reaction
• What is purpose of temperature cycling
• Basic PCR experimental design