Techniques of molecular genetics

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Techniques of molecular genetics

• Jonathan Wolfe
• website http//www.ucl.ac.uk/ucbhjow/index.html

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Cloning

• cDNA clones
• Expression studies
• Protein structure and function studies
• Full length (or near full length) cDNAs can be
  sequenced for comparison with the genomic
  sequence.
• Genomic clones
• Gene structure, function and evolution studies

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Some genomic cloning vectors
Cloning Vectors
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Electrophoresis
This technique can be used to separate any
molecules which differ in either M.Wt. or charge.
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Electrophoresis

• For example
• Agarose gel electrophoresis

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Southern Blotting
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Southern Blotting
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Southern Blotting
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Southern Blotting
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Southern Blotting
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Polymerase chain reaction (PCR)
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Polymerase chain reaction (PCR)
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DNA Sequencing
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DNA sequencing
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DNA sequencing
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Reminder

• Visit me athttp//www.ucl.ac.uk/ucbhjow/index.h
  tml