Using a Polarographic System to Study Mitochondria

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Using a Polarographic System to Study
Mitochondria
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Mitochondria in situ
mitochondria
Rattus norvegicus, cardiac myocyte
Dictyostelium discoideum
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Mitochondria Structure
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Why Measure Dissolved Oxygen?
Intermembrane space
protons
protons
protons
electrons
oxygen electrons protons
Matrix
water
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Polarographic System
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Clark Electrode
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How the Electrode Works
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Filling and Calibration
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Adding Mitochondria
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Troubleshooting
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Injecting Reagent
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Recording the Results