Research preparedness for emerging infectious diseases

1
Research preparedness for emerging infectious
diseases

• HKU
• Health, Welfare and Food Bureau
• Investigators
• Yuen KY, Peiris JSM, Guan Y, Woo PCY, Lau SKP,
  Kao YT, Zheng BJ, Poon LLM, Chen HL, Wong SSY, Ho
  PL, Chan KH, Yam WC.

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Clinical microbiologist(MD,MRCPath)
Laboratory(P3 animal)
Hospital wards Patients
Basicscientists(PhD)
Ward consult

Lab report

Phone consult
ID Problems

Infection control,HA / CHP
Clinician(MD)
Epidemiology, School of Public Health / CHP / DH
/ WHO
3
Investigation of avian influenza by a clinical
team from HKSAR on behalf of WHO
Collection of clinical data, formulation of
clinical guideline for patient management.
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(No Transcript)
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Research projects supported by RFCID

• Ecology of SARS virus / influenza virus /
  bacteria / antimicrobial resistance in animals
  and human.
• The role of viral surface protein in mediating
  the cross species transmission.
• Pathogenicity test of different virus / bacteria
  isolates from animals.
• Prediction of the next candidate for an epidemic
  of SARS / flu.
• Development of rapid diagnostic systems by
  genetic amplification / microarray, antigen
  detection and antibody test for these possible
  candidate.
• Development of candidate vaccines and
  antimicrobials.

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Clinical characteristics of SARS
Lancet 2003
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Foetal rhesus kidney (FRhK-4) cells and to grow
the virus
Cell culture with CPE
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Lancet 2003 Apr
9
Second generation diagnostic tests
Light cycler / Tagman hybridization
ELISA WB IF
Clin Chem 2003
J Clin Microbiol 2004
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Ribavirin Kaletra
Ribavirin
Number at risk Treatment arm 41 41 41 41 41 Histor
ical arm 111 111 103 91 85
Thorax 2004
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Chlorogenic acid
Glycyrrhizin
Sheng Gan Cao (Glycyrrhiza uralensis)
Jin Yin Hua (Flos lonicerae)
Baicalin
Artemisinin(artensunate)
Huang Qin (Scutellaria baicalensis)
Qing Hao (Artemisia apiacea)
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Checkerboard assay for synergism between
interferons and ribavirin by neutralization test
without pre-incubation
leukocytic
leukocytic
J Clin Virol 2004
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Phylogenetic analysis of S gene of human and
animal SARS viruses
SZ1
Animal
SZ13
MHV
SZ16
SZ3
GZ50
CUHK-W1
HKU36871
HKU39848
HKU66078
Human
HKU65806
Urbani
Tor2
GZ01
GZ60
GZ43
100 nucleotide
Science 2003
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One year after SARS

• Biochem Biophys Res Commun 2004 Mar
  19315(4)1134-9.
• Clin Chem 2003 Jun49(6 Pt 1)953-5.
• Clin Chem 2004 Jan50(1)67-72.
• Clin Chem 2004 Mar 30.
• Clin Exp Immunol 2004 Mar135(3)467-73.
• Clin Infect Dis 2004 Feb 1538(4)467-75.
• Clin Infect Dis 2004 Jan 1538(2)297-9.
• Emerg Infect Dis 2004 Feb10(2)176-8.
• Emerg Infect Dis 2004 Feb10(2)294-9.
• Emerg Infect Dis 2004 Feb10(2)342-4.
• Exp Biol Med (Maywood) 2003 Jul228(7)866-73.
• Gastroenterology. 2003 Oct125(4)1011-7.
• J Biol Chem 2003 Oct 10278(41)39578-82.
• J Clin Microbiol 2003 Oct41(10)4521-4.
• J Clin Virol 2003 Dec28(3)233-8.
• J Clin Virol 2003 Dec28(3)245-7.
• JAMA 2003 Nov 26290(20)2665-6.

• Lancet 2003 Apr 19361(9366)1319-25.
• Lancet 2003 Jul 26362(9380)263-70.
• Lancet 2003 May 10361(9369)1615-7.
• Lancet 2003 May 24361(9371)1767-72.
• Lancet 2003 May 24361(9371)1773-8.
• Lancet 2003 May 3361(9368)1519-20.
• Lancet 2003 Nov 29362(9398)1807-8.
• Lancet 2003 Oct 25362(9393)1353-8.
• Lancet 2004 Jan 10363(9403)99-104.
• Lancet 2004 Mar 13363(9412)841-5.
• N Engl J Med 2003 Dec 18349(25)2431-41.
• N Engl J Med 2003 May 15348(20)1977-85.
• Nature 2003 May 15423(6937)240.
• Nature 2003 Oct 30425(6961)915.
• Respirology 2003 Nov8 SupplS6-8.
• Science 2003 Oct 10302(5643)276-8.
• Thorax 2004 Mar59(3)252-6.

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Patrick Manson, 18441922, father of tropical
medicine. 1878 filariae in elephantiasis
through Culex mosquito. 1894 deduced malaria
passing through mosquito. 1887 founded Hong Kong
College of Medicine for Chinese. (HKU)
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Professor CT Huang, 1976.
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Protecting China from emerging infectious
diseases and bioterrorism
SARS, estimated economic lossChina US 6.1
billion, HKSAR 2.1 GDP (Asian Development Bank)