Thrombin

1
Adaptamers
Thrombin-binding domain
Streptavidin-binding domain
Linking region
Thrombin
Streptavidin
2
Why develop adaptamers?

• To offer a new way to target substrates to cells.
• To create a tool for studying cell-cell
  interactions.
• To provide a foundation for engineered catalysts.

3
Adaptamer testing
streptavidin
adaptamer
Adaptamer A15
thrombin
anti-thrombin antibody
Control no thrombin
Bead surface
4
Adaptamer quenching
Displacement strand

Results
No displacement strand added
Displacement strand added
Background fluorescence