Lab Exercise 29A Blood Typing

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Lab Exercise 29ABlood Typing
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Step 1 Drawing Blood OUCH!Be VERY clean! Use
the alcohol to clean your finger and use a brand
new lancet everytime! Dispose of lancet in
Sharps container when finished.
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Step 2 Squeeze out a nice drop of blood
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Heres what the blood typing card looks like
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Step 3 The Procedure

• Place a drop of blood on the right side of each
  circle group.
• Place a drop of antibodies on the left side of
  each circle group.
• Anti-A goes in the 1st circle
• Anti-B goes in the 2nd circle
• Anti-Rh goes in the 3rd circle

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The Antibodies
Blue Anti-A Yellow Anti-B White Anti-Rh
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Step 4 Mixing the antibodies with the blood

• Mix the antibodies with the blood and look for
  the agglutination reaction.

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Recall

• Each antibody will attack cells containing their
  SPECIFIC surface antigen.
• Anti-A will ONLY attack cells containing surface
  antigen A
• Anti-B will ONLY attack cells containing surface
  antigen B
• Anti-Rh will ONLY attack cells containing the Rh
  surface antigen
• If the antibody attacks the cell it causes
  agglutination or clumping of the cells.

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• In this test, we are adding specific antibodies
  to each drop of blood.
• If we see agglutination with a particular
  antibody, we can conclude that the blood cells DO
  contain that surface antigen.
• Lets take a look at my results

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My results Can you tell whats my blood type????
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Did Anti-A cause agglutination?
NO! Conclusion My blood has NO surface antigen
A
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Did Anti-B cause agglutination?
NO! Conclusion My blood has NO surface antigen
B
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Did Anti-Rh cause agglutination?
YES! Conclusion My blood HAS surface antigen Rh
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Results No surface antigen ANo surface
antigen BYes I have surface antigen Rh
I am blood type O
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Tips and Tricks

• If you are a poor bleeder massage the blood
  into the tip of your finger before drawing blood.
• When drawing blood push the lancet device into
  your finger and you release the trigger.
• All trash goes into the RED SHARPS CONTAINER!

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