Title: Can Halorabdus utahensis secrete proteins?
1Can Halorabdus utahensis secrete proteins? A.
Malcolm Campbell
2Halorabdus utahensis habitat
3Halorabdus utahensis habitat
4- 28 w/v NaCl in Gunnison Bay excludes many
species - Brine shrimp reproduce in low salt areas of
Gunnison Bay - Brine shrimp feed on algae with cell walls of
cellulose and other - complex sugars
- Shrimp in main body of Gunnison Bay die
- Brine flies inhabit the shoreline
http//ut.water.usgs.gov/greatsaltlake/
5Translation with Secretion
Albert Bolhuis. 2004. Phil. Trans. R. Soc. Lond.
B. Vol. 359 919927.
6Translation with Secretion
Signal peptidase I (EC 3.4.21.89)
Albert Bolhuis. 2004. Phil. Trans. R. Soc. Lond.
B. Vol. 359 919927.
7Translation then Secretion
Tat proteins use protein domain of SRRXFLK to be
targeted to be exported
Figure from Sonja-Verena Albers, Zalán Szabó and
Arnold J. M. Driessen. 2006. Nature Reviews
Microbiology. VOLUME 4.
8Translation then Secretion
Tat proteins use protein domain of SRRXFLK to be
targeted to be exported
Figure from Sonja-Verena Albers, Zalán Szabó and
Arnold J. M. Driessen. 2006. Nature Reviews
Microbiology. VOLUME 4.
9Translation then Secretion
Tat (twin arginine targeting proteins) use
protein domain of SRRXFLK to be targeted to be
exported
? cellulase (glycosyl hydrolase family 5)
MTDPDRPPTGDREASQSNTTTGGEGPSRRTFLK ?
phage tail protein, P2 protein I family
MTRRTNDTGEVDEKPSSGAEQQGSNDSTGSRDPSRRDFLK... ?
candidate polyfunctional acetylxylan
esterase-B-xylosidase-a-L-arabinofuranosidase,
glycoside hydrolase Family 43 protein and
Carbohydrate Esterase Family 6 protein
MTRRTNDTGEVDEKPSSGAEQ
QGSNDSTGSRDPSRRDFLK ? Endoglucanase
MTHNNPDDDSTARRTTESTESPSTAGIASASRR
DFLK... ? Exocellobiohydrolase A/1,4-beta-cellobio
hydrolase A
MTHNNPDDDSTARRTTESTESPSTAGIASASRRDFLK ? Cellulase
(glycosyl hydrolase family 5)./Fibronectin type
III domain
MTDEATESIEASATDHTDETAGNRKDPGLTSSRRTFLG...
10