Can Halorabdus utahensis secrete proteins? - PowerPoint PPT Presentation

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Can Halorabdus utahensis secrete proteins?

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Brine shrimp reproduce in low salt areas of Gunnison Bay ... Tat (twin arginine targeting proteins) use protein domain of SRRXFLK to be ... – PowerPoint PPT presentation

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Title: Can Halorabdus utahensis secrete proteins?


1
Can Halorabdus utahensis secrete proteins? A.
Malcolm Campbell
2
Halorabdus utahensis habitat
3
Halorabdus utahensis habitat
4
  • 28 w/v NaCl in Gunnison Bay excludes many
    species
  • Brine shrimp reproduce in low salt areas of
    Gunnison Bay
  • Brine shrimp feed on algae with cell walls of
    cellulose and other
  • complex sugars
  • Shrimp in main body of Gunnison Bay die
  • Brine flies inhabit the shoreline

http//ut.water.usgs.gov/greatsaltlake/
5
Translation with Secretion
Albert Bolhuis. 2004. Phil. Trans. R. Soc. Lond.
B. Vol. 359 919927.
6
Translation with Secretion
Signal peptidase I (EC 3.4.21.89)
Albert Bolhuis. 2004. Phil. Trans. R. Soc. Lond.
B. Vol. 359 919927.
7
Translation then Secretion
Tat proteins use protein domain of SRRXFLK to be
targeted to be exported
Figure from Sonja-Verena Albers, Zalán Szabó and
Arnold J. M. Driessen. 2006. Nature Reviews
Microbiology. VOLUME 4.
8
Translation then Secretion
Tat proteins use protein domain of SRRXFLK to be
targeted to be exported
Figure from Sonja-Verena Albers, Zalán Szabó and
Arnold J. M. Driessen. 2006. Nature Reviews
Microbiology. VOLUME 4.
9
Translation then Secretion
Tat (twin arginine targeting proteins) use
protein domain of SRRXFLK to be targeted to be
exported
? cellulase (glycosyl hydrolase family 5)
MTDPDRPPTGDREASQSNTTTGGEGPSRRTFLK ?
phage tail protein, P2 protein I family
MTRRTNDTGEVDEKPSSGAEQQGSNDSTGSRDPSRRDFLK... ?
candidate polyfunctional acetylxylan
esterase-B-xylosidase-a-L-arabinofuranosidase,
glycoside hydrolase Family 43 protein and
Carbohydrate Esterase Family 6 protein
MTRRTNDTGEVDEKPSSGAEQ
QGSNDSTGSRDPSRRDFLK ? Endoglucanase
MTHNNPDDDSTARRTTESTESPSTAGIASASRR
DFLK... ? Exocellobiohydrolase A/1,4-beta-cellobio
hydrolase A
MTHNNPDDDSTARRTTESTESPSTAGIASASRRDFLK ? Cellulase
(glycosyl hydrolase family 5)./Fibronectin type
III domain
MTDEATESIEASATDHTDETAGNRKDPGLTSSRRTFLG...
10

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