Chapter 8 Addedum Microarrays

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Chapter 8 (Addedum) - Microarrays

• Microarrays

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What are microarrays?

• Small silicon chip or microscope slide
• Short pieces of __________________ are stamped
  onto slide in a specific pattern
• Must know what

Silicon chip
Spot containing short segment of a gene
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How to set up a microarray

• Isolate ________ from 2 samples
• Use
• mRNA ? cDNA
• Fluorescent label on each sample of cDNA with
  different colors (ex red and green)
• Mix samples and allow
• cDNA will bind to complementary bases only
• cDNA binding represents
• Compare expression patterns in 2 samples

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Microarrays
Nucleotide Microarray