AntiViral Functions of Mucosal IgA Antibodies

1
Anti-Viral Functions of Mucosal IgA Antibodies

• Michael E. Lamm
• Institute of Pathology

2
(No Transcript)
3
(No Transcript)
4
(No Transcript)
5
Paramyxovirus
6
(No Transcript)
7
(No Transcript)
8
(No Transcript)
9
(No Transcript)
10
(No Transcript)
11
(No Transcript)
12
(No Transcript)
13
(No Transcript)
14
(No Transcript)
15
(No Transcript)
16
Intracellular neutralization of HIV by
transcytosing IgA against internal HIV-1
proteins. a
a Polarized monolayers of pIgR or pIgR- Vero
C1008 cells were transfected with HIV proviral
DNA for 4 h and then exposed to MAbs
basolaterally for 18 h. HIV levels in apical
supernatants and cell lysates 30 h post
transfection were determined by infecting target
HeLa cells (blue cell assay). Data are means
from eight data points ? SEM pooled from four
experiments. b Compared to the no antibody
control. c Result significantly different
(plt0.005) from the no antibody control according
to a two-tailed t-test.
17
Direct measurement of p24 concentrations. a
a Experiments as in Table 1 except that p24
concentrations were measured. MAbs were added
for 18 h to the basolateral surface of polarized
monolayers of pIgR Vero C1008 cells that had
been transfected with HIV proviral DNA for 4 h.
HIV levels in apical supernatants, basolateral
media and cell lysates 30 h post transfection
were determined by p24 ELISA assay. Data are
means from 11 data points ? SEM pooled from seven
experiments. b Compared to the no antibody
control. c Mean virus titer significantly less
than control (plt0.02).
18
(No Transcript)
19
Model of HIV assembly and budding
20
(No Transcript)
21
(No Transcript)
22
(No Transcript)
23
(No Transcript)
24
(No Transcript)
25
(No Transcript)
26
(No Transcript)
27
(No Transcript)
28
(No Transcript)
29
(No Transcript)
30
(No Transcript)
31
(No Transcript)
32
(No Transcript)
33
(No Transcript)
34
(No Transcript)
35
(No Transcript)
36
(No Transcript)
37
Immunofluorescence analysis of kidney sections
from mice injected with IgA immune complexes and
then with IgA protease.
aValues are mean (?
se) normalized fluorescence intensity (saline
control 100) pooled from four independent
experiments b F?11.3, plt0.001 vs saline treatment
c F?11.3, plt0.001 vs no immune complexes
38
Virus Studies
Excretion In Vivo

• Yung Huang
• Mary Mazanec
• John Nedrud
• Alison Wright
• Huimin Yan

• Thomas Blanchard
• Janet Robinson

IgA Nephropathy

• Steven Emancipator
• Andrew Plaut
• Janet Robinson
• Jiazhou Qiu