Miniature Practical Organic Chemistry

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Miniature Practical Organic Chemistry
Date 7 August 2008 Time 900 am - 500
pm Venue LT-H and SynLab, Room 6124,

• UST and EDB

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Why Microscale Chemistry?

• Reduction of chemical use
• Environmental Friendly
• More economical
• Smaller is safer!

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Todays Programme
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Floor Plan
6124 Entrance
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Synthetic Laboratory 6124
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Synthetic Laboratory 6124
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Experiment 1 Apparatus
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Experiment 1 Chemicals
Conc. sulfuric acid is very corrosive !
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Experiment 1 Reflux Setup

• Things to Remember
• Heat the heating block earlier
• Be careful of the hot surface, especially the
  heating block!
• Dont melt the rubber tubings
• Use a spatula to regulate the sand level, i.e. to
  control the reflux temperature

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Other Precautions of Expt. 1

• After reflux, the reaction mixture must be cooled
  down to room temperature before neutralize with
  NaHCO3
• NaHCO3 must be added slowly to the reaction
  mixture for neutralization
• For extraction, after shaking the reaction
  mixture with organic solvent, the cap of the test
  tube must be released or open so as to avoid
  building up of pressure

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Experiment 2 Apparatus
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Spinach After Extraction
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Extraction of Chlorophyll
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Thin Layer Chromatography
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Evaporation of Solvent
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Column Chromatography
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Column Packing
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Column Sample Loading