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Institut of plant biochemistry. L interaction non-h te Persil-Phytophthora sojae, ... Institut of plant biochemistry. GP42 TGase homologs are highly conserved among ... – PowerPoint PPT presentation

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1
Linteraction non-hôte Persil-Phytophthora sojae,
un modèle pour étudier la réponse immunitaire
innée chez les plantes
Institut of plant biochemistry
2
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5
Pep-13, a minimal elicitor motif
within GP42
Elicitor-active
oligopeptide
Open reading frame
X
P
H
C
C
C
RACE clones
X
H
N
cDNA clone
VWNQPVRGFKVYE
Elicitor activity

-

polypeptides
-
expressed from
subclones
-

-
TS
-
TSGPTS
-
Institute of Plant Biochemistry
6
Institut of plant biochemistry
7
GP42 TGase homologs are highly conserved among
the genus Phytophthora
Institute of Plant Biochemistry
8
Pep-13-containing GP42 TGase homologs are highly
conserved among Phytophthora spp.
Institute of Plant Biochemistry
IPB
9
Hydropathy plot of Phytophthora sojae
transglutaminase
Pep-13 is part of a surface-exposed loop structure
Institute of Plant Biochemistry
IPB
10
TGase and elicitor activities of P. sojae
wild-type and mutant proteins Pep-13 is
functionally indispensable

Institute of Plant Biochemistry
IPB
11
Pep-13 elicits defense reactions in potato with
the same signal specificity as in parsley
P234A
W231A
Institute of Plant Biochemistry
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