Procedure for plasmid purification

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Procedure for plasmid purification
96 x 11.25 ml E. coli bacterial culture in
96-Deepwell Culture Plate Centrifuge Deepwell
Culture Plate. Pour off or aspirate supernatants
Add Resuspension buffer. Add Lysis buffer.
Add Neutralization buffer Pipette each lysate
in the Culture Plate to Filter Plate A. Apply
vacuum. Add DNA Binding Buffer to Filter Plate
DB (on top of Manifold). Apply vacuum . Add
Washing buffer to Filter Plate DB. Apply
vacuum. Add Molecular Biology Grade Water to DNA
binding matrix in Filter Plate DB.
Incubate. Gradually apply vacuum to elute plasmid
DNA into Collection Plate Result purified
plasmid DNA in Collection Plate
Place Filter Plate A on top of Filter Plate
DB Place Filter Plate DB on top of
Collection Plate