Genetic%20mapping%20using%20a%20Rat%20Heterogeneous%20stock

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Genetic mapping using a Rat Heterogeneous stock
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Heterogeneous stocks
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Heterogeneous stocks
Pseudo-random mating for 50 generations
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Heterogeneous stocks
Pseudo-random mating for 50 generations
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Low resolution mapping in an F2 intercross
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High resolution mapping in HS
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Covariates

• Age
• Apparatus
• Number of animals in a cage (cage density)
• Experimenter
• Litter size
• Season
• Month
• Hour

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Can we do the same in rats?

• BN/SsN
• MR/N
• BUF/N
• M520/N
• WN/N
• ACI/N
• WKY/N
• F344/N
• C Hansen K Spuhler, 1984

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F2 intercross data from rats
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HS high resolution data
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High throughput phenotyping
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Samples

• Blood sample naïve rats
• Full blood count (EDTA treated blood),
• FACS (heparin treated),
• LPS response (heparin treated)
• Serum sample at time of sacrifice (1.5ml)
• Tissues heartaorta, spleen, kidney, liver,
  brain, spinal cord, ears, thymus, tail and
  adrenal glands.

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IGS data base
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