Cell Theory and Cell Study: Tools and Techniques

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Cell Theory and Cell Study Tools and Techniques
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The Dissecting Microscope

• Allows the study of large specimens.
• It has two eyepieces and two objectives.
• It has low power magnification and 3D quality.
• It is also called a Stereo-microscope.

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Do Now!

• Answer questions in UPCO page 54 1-3.

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The Electron Microscope

• Can magnify up to 100,000x.
• Cannot use live specimens.
• Use a narrow beam of electrons instead of light.
• Magnets are used to focus the electron beam.

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Do Now!

• Answer questions 1-3 on pages 54,55.

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The Centrifuge

• Instrument used to separate cell parts.
• Separate parts according to density.
• Material is spun around in a test tube at very
  high speeds.
• Heaviest material is at the bottom, lightest is
  at the top.

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Do Now!

• Answer question 1 in the middle of page 55.

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Staining Techniques

• Many parts of the cell are colorless and hard to
  see.
• Staining techniques color certain cell parts so
  they can be more easily studied.
• The most commonly used stains are Methylene Blue,
  and Iodine (Lugols Solution).

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Do Now!

• Answer questions in UPCO page 53, 1, 2 .

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Magnification

• Compound microscopes- each lens enlarges the
  image.
• Total magnification is determined by multiplying
  the power of the eyepiece lens x the power of the
  objective lens.
• Total magnification refers to the total amount
  the image is enlarged.
• The unit of length used to measure specimens is
  the micrometer (u).

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Do Now!

• Answer questions 1-4 on page 55,56 in UPCO.
• Answer questions 1,2 in the middle of page 56.