Fischer at al' J Immunol Methods 2002, 259:15969

1
Cytotoxicity
CTL
PKH-26
Fischer at al. J Immunol Methods 2002,
259159-69 Fig. 3. Analysis of specific
cytotoxicity using the PKH-26 assay. Dot plot of
ann-FITC/PI flow cytometry of PKH-26 gated
Melan-A-expressing target cells after
coincubation with Melan-A-specific CTL at
different ErT ratios
2
In-situ Hybridisation
PCR
IN-SITU HYBRIDISATION
Patterson at al. J Virol 1995, 69
43164322. Fig. 2. Comparison of representative
two-color dot plots of monocyte-depleted PBMCs
from patients with varying CD4
3
Nuclear translocation
NP-40
Blaecke et al. Cytometry 2002, 48 7179 Fig. 6.
Measurement of NFB translocation by flow
cytometry. Dose-response curve of NF-B
translocation after 30 min of LPS treatment of
human DCs. Results are expressed as the mean
values of MFI, taken from FACS histograms and the
SDs were obtained from five experiments
4
Binding Internalization
de Boer EC et al. Cytometry 1996 25 381-7 Fig.
1. Green (El, X-axis) vs. red (FL2, Y-axis)
fluorescence, showing BCG internalization and
binding by T24 bladder carcinoma cells.
Incubation of R4 cells with fluoresceinated BCG
(10 clu BCG/cell) for a duration of 4 h and
subsequent labeling with anti-BCG and
PE-conjugated second antibody FLl m2- cells
(quadrant 4) represent BCG-internalizing cells,
FLl/FL2 cells (quadrant 3) represent
BCG-binding cells.
5
Isolation of genes from libraries
Griffiths at al. EMBO J. (2003) 22 2435 Fig. 5
After fluorescent labelling using anti-product
antibodies the beads were analysed by flow
cytometry. The levels of fluorescence (FL1-H) on
single unsorted beads are plotted as histograms.
The 'positive' highly fluorescent beads (in
region M1) were sorted from the 'negative'
low-fluorescence beads and re-analysed. The genes
on the sorted 'positive' beads (and on unsorted
beads) were PCR amplified and analysed by agarose
gel electrophoresis
6
DNA dommage
TdT- mediated dUTP nick end labelling
TUNEL
DAPI
7
Cytokines dosage
8
Detection of mRNA