Indirect Labeling Tyramide Signal Amplification

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Indirect Labeling (Tyramide Signal Amplification)
Anti-FL-HRP binds to Hybridized FL labeled cDNA
Isolate total RNA
Reverse Transcribe using FL or Biotin-labeled
oligo-dT primers
HRP catalyzes deposition of cyanine 3 tyramide
Hybridize to microarray
HRP is inactivated
Streptavidin-HRP binds to hybridized biotin
labeled cDNA
TSA detection
HRP catalyzes deposition of cyanine 5 tyramide
Imaging
Schematic IV