Optimization of Aquaponics System in Hawaii: Preliminary data

1
Optimization of Aquaponics System in Hawaii
Preliminary data

• Marissa Lee
• University of Hawaii, Manoa College of Tropical
  Agriculture and Human Resources
• Department Molecular Bioscience and
  Bio-Engineering
• November 21, 2009
• Bilger Hall 150

2
Presentation Agenda

• Project Rationale
• Experiment 1 Effects of Lighting
• Shade
• No Shade
• Experiment 2 Effects of Different Feeds
• Protein Content
• Fat Content

3
Project Rationale

• Partial fulfillment of MBBE graduation
  requirement
• Senior thesis
• Partial fulfillment of CTAHR vision and mission
• Generate and transfer science based information
  for stake holders to make informed decisions.

4

• Experiment 1 Test the effects of light
• How are nutrients in fish effluent affected?
• How are the fishs growth affected?
• What is the crop output per treatment?

5
The experiment was held at UH Freshwater
Aquaculture Facility at Windward Community College
6
Experiment 1

• Fish Oreochromis sp.
• Plant Lactuva sativa- Red sail Lettuce

7
Experiment 1Test the effects of light- Two
treatments

• Treatment 2

• Treatment 1

8
Experimental Design Tank Setup

• Each tank stocked with 323 Tilapia.
• Average body weight 1.64g
• Stocking density 0.28 kg/m3
• Salmon Starter Feed
• 52 crude protein
• 16 crude fat

• Biofilm

9
Lettuce Trays
Flow rate 1.35 seconds to fill 1 Liter
10
Air Lift
11
Experiment 1
12
Temporal Changes in Dissolved Oxygen in Covered
and Uncovered Tanks
p lt 0.01 Difference is statistically significant
13
Temporal Changes in pH in Covered and Uncovered
Tanks
p lt 0.01 Difference is statistically significant
14

• Turbidity tube
• ? measuring the amount of particles.

p lt 0.01 Difference is statistically significant
15
(No Transcript)
16
P lt 0.0001 Difference is statistically
significant
17
(No Transcript)
18
Summary Experiment 1
19
Results Experiment 1
20

• Experiment 2 Effects of different feeds
• How are nutrients in fish effluent affected?
• Is fish growth affected?
• Is crop out put affected?

21
Experiment 2

• Fish Oreochromis sp.-Tilapia

Plant Lactuva sativa- Red sail Lettuce
Plant Brassica juncea- Mustard Cabbage
Plant Lactuva sativa- Manoa Lettuce
22
Experiment 2Two treatments
Silver Cup
Rangen 350

• Treatment 1- Rangen 350

• Treatment 2- Silver Cup

23
Different Treatments

• Rangen
• Crude Protein35.0
• Crude Fat.5.0
• Crude Fiber..5.0
• Ash..10.0
• Phosphorus..1.0

• Silver Cup
• Crude Protein.45.0
• Crude Fat...16.0
• Crude Fiber..3.0
• Ash..12.0
• Phosphorus..1.2

0.77/ lb
0.63/ lb
24
Experiment Design Tank Setup

• Each tank was stocked with 200 Tilapia
• Tank average 26.4 g
• Stocking density 2.78 kg/ m3

• Bioflim

25
Experiment 2
26
Water Quality
27
Trace Minerals in Water

• Rangen
• N 14.9 ppm
• P 1.0
• K 10.6
• Ca 6.0
• Mg 5.1
• Na 10.9
• Fe 0.1
• Mn 0.1
• Zn 0.04
• Cu 0.01
• B 0.03

• Silver Cup
• N 17.8 ppm
• P 1.9
• K 7.7
• Ca 9.8
• Mg 5.0
• Na 6.3
• Fe 0.1
• Mn 0.1
• Zn 0.1
• Cu 0.0
• B 0.0

Agricultural Diagnostic Service, supplmented
28
Analysis of Covariance p gt 0.05 not
statistically different
T-Test for last sampling day p gt 0.05 not
statistically different
29
p lt 0.05
Silver Cup
Rangen
30
plt 0.01
Silver Cup
Rangen
31
pgt0.05
Silver Cup
Rangen
32
Potassium Hydroxide
33
Summary Experiment 2
34
Experiment 2 Summary
35
Acknowledgements

• Clyde Tamaru
• Kathy McGovern-Hopkins
• Harry Ako
• Mom and Dad
• My friends