ECE-1466 Modern Optics Course Notes Part 8

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ECE-1466Modern OpticsCourse NotesPart 8

• Prof. Charles A. DiMarzio
• Northeastern University
• Spring 2002

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Phase Microscopy

• Differential Interference
• Phase Contrast
• True Phase Microscopy
• Phase Reconstruction from Amplitude

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Phase Contrast Microscopy
F
F
F
F
A
A
Obscuration in Pupil Plane Removed DC and Low
Spatial Frequencies
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Phase Contrast 1-D Example
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Phase Contrast 2-D Example
Image Plane
Pupil Plane
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Differential Interference Contrast
Polarizing Cube at Illumination Lens Splits Beam
and Another Polarizing Cube at the Objective Puts
it Back Together.
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DIC 1-D Example
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Quadrature Imaging Concept
Quadrature
Balanced Mixing
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How Could QTM Count Cells in Compact Embryo?
QTM Phase
DIC
Assumed Index Profile Before Compaction
M10026.m
M10026.m
Assumed Index Profile During Compaction
M10026.m
M10026.m
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Phase Retrieval from Amplitude
Measure Irradiance at Plane A
Measure Irradiance at Plane B
Guess Field at Plane A
Object
A
B
Keep Phase, and Change Amplitude to Match the
Measurement
Compute Field at Plane B
Keep Phase, and Change Amplitude to Match the
Measurement
Compute Field at Plane A