ImageJ analysis:

1
ImageJ analysis
Run vertical plot-profile to find average image
brightness on a path perpendicular to the
wire Profile exported as gray value v.
distance in Excel format
2
MATLAB analysis
Wire data removed data above and below wire
averaged, normalized Script calculations (1)
locate all negative derivatives (2) find
midpoint of the negative
derivatives vector (3) find average
brightness from midpoint to end this is the
background value (4) mark index when
brightness data is within 10 of background
value (5) scarring distance index (6)
scarring index integral from 0 to index of (1
ynormalized)