Impact and Efficacy of Aerial ULV Application of Permanone 3030

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Impact and Efficacy of Aerial ULV Application of
Permanone 30-30
Research Report
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Objectives

• Measure permethrin residue ground deposition
• Determine permethrin airborne concentration
• Assess non-target impact using mosquito fish
  bioassay
• Evaluate control efficacy against adult
  mosquitoes through bioassay
• Correlate residue and bioassay

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Materials and Methods

• Study Site Panama City Beach
• 12 stations (9 treatments and 3 controls)
• The 9 treatment stations include 6 open sites and
  3 in woodland area
• Beach MCDs helicopter (Helo N91336) equipped
  with WingmanTM GX and AIMMS-20 (Adapco) was used
  for the Aerial ULV spray at 150ft attitude.
• Residue sample collections yarn, water and
  filter paper
• Permethrin Analysis -GC with ECD detector

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Materials and Methods

• Bioassay caged mosquitoes (50 female mosquitoes
  / cage) (Ochlerotatus taeniorhynchus) 12-hrs
  post-treatment
• Bioassay caged mosquito fish (Gambusia
  holbrooki) in plastic pans (10/pan). The acute
  toxicity was checked 1 hr after the spray and the
  chronic toxicity was assessed daily for 5 days in
  the same plastic pan (108-hrs post-treatment
  --worst case scenario)

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Result (1) Permethrin Residue on Filter Paper
(µg/m2) and (Fish Mortality)
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Result (2) Permethrin Residue on Yarn (µg/yarn)
and (Mosquito Mortality)
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Discussion Permethrin Residue

• Low level permethrin residue (2.14 ppb and 4.36
  ppb) was detected in water samples from the fish
  tank from two separate spray events (6/27/2006
  and 7/18/2006) and both samples collected from
  site 1
• The highest permethrin deposition on filter paper
  was on site 9 (151µg/m2). That matched up to low
  residue on yarn (6.0 µg/yarn) indicating it may
  have the large droplets fall out. In fact that
  many of higher ground deposition (gt90 µg/m2) did
  not have good mosquito control efficacy
• The range of ground deposition was about 37 151
  µg/m2
• The highest permethrin residue found on yarn was
  170 µg/m2 and the range was 3 170 µg/m2
• With few exceptions, the residue on yarn reached
  gt40 µg/yarn, the control efficacy was greatly
  improved.

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Discussion (2) fish and mosquito assay

• PPB level permethrin residue was detected in tank
  water samples from two separate spray events on
  site 1 did not cause fish mortality at higher
  concentration (4.36 ppb).
• There were no indication of acute toxic effect
  permethrin on fish from field bioassay even with
  the highest ground deposition (151 µg/m2).
• Various fish mortality found during each trial
  and the reason is unknown or appears not
  correlated with permethrin concentration
• We suspect the stressed fish were picked for the
  assay in trial 2 and 3 as the control fish
  mortality increasing. The fact of fish reservoir
  lost screen enclosure as a result from storm.
• Only six stations in three spray evens reached
  gt80 mosquito mortality. The low control efficacy
  in general a concern and the reasons need to be
  further investigated.

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Acknowledgements

• The following personnel contributed to this
  project
• Florida AM University
• C. Brock, T. Quimet, T. Lian, R. Aarons, N.
  Sickerman, -participation
• K. Shaffer and J. Petersen Provide mosquito
  pupae
• J. Smith, E. Cope and J. Walsh Provide mosquito
  rearing room
• Beach Mosquito Control Districts
• E. Hunter, J. Shehee, Skip, Lee, and Cindy -
  participation
• Florida Department of Agricultural and Consumer
  Services
• Max Feken, James Clauson -participation
• Bayer Environmental Science
• Peter Connelly -participation, Jing Zhai
  (consultant)
• Adapco,
• Bill Reynolds computer model and spray tech.
• Lee County Mosquito Control District
• J. Hornby droplets analysis

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Any Questions ?