Effect of prolonged mechanical ventilation on diaphragm

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Effect of prolonged mechanical ventilation on
diaphragm muscle mitochondria in piglets
In collaboration with Peter Radell and Lars I
Eriksson Dep. Anesthesiology and Intensive Care
Karolinska University Hospital in Solna
Katarina Fredriksson
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Background
Muscle weakness in ICU patients Problems with
weaning Possible mechanism mitochondrial
derangements
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Overall aim
To evaluate the effects of mechanical ventilation
on mitochondrial function and markers of
oxidative stress in diaphragm muscle from piglets.
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Piglets Ref. Radell et al. Intensive Care Med
2002

• Age 2-4 months
• Weight 23-30 kg
• Anesthesia ketamine
• No muscle relaxants used

• Control animals
• N8
• Mechanically ventilated
• for 4-6 hours

• Ventilated animals
• N7
• Mechanically ventilated
• for 5 days

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Radell et al. Intensive Care Med 2002
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Aim 1 To look at changes in mitochondrial content
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Electron microscopic picture of diaphragm muscle
from piglets
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Aim 2 To evaluate mitochondrial function
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Aim 3 To evaluate the extent of oxidative stress
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Superoxide dismutase
2O2- 2H Superoxide dismutase H2O2
O2
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Glutathione
GSH reduced glutathione GSSG oxidized
glutathione GPX Glutathione peroxidase
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Conclusion
The impairment seen in diaphragm muscle function
after 5 days of mechanical ventilation in piglets
is not associated with derangement in
mitochondria.