VVP Fig 733

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VVP Fig 7-33
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Fv
Fab
binding sites
Fc
IgG molecule
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link
http//www.wiley.com/college/fob/quiz/quiz07/7-29.
html
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Enzymes Chapter 11
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VVP Fig 11-1 Trypsin inhibitor
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VVP Fig 11-5
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Orientation
Hexokinase VVP p 386
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Enzymatic catalysis proceeds by one or more of
1) general acid/base catalysis (GABC) 2)
covalent catalysis 3) electrostatic
stabilization 4) proximity effects 5)
preferential stabilization of the
Effects 1 and 3 are often manifested as effect
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(uncatalyzed)
VVP Fig 11-6
(general acid)
(general base)
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His12
His119
VVP Fig 11-7 RNase S
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VVP Fig 11-8
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General Base
General Acid
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General Acid
General Base
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Covalent catalysis
Is characterized by the formation of a covalent
Enz-S adduct that alters the reaction
pathway Nucleophiles many amino acid
sidechains (H, K, C, S, D, E, Y), some cofactors
(TPP) Electrophiles some cofactors (e.g. PLP)
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VVP Fig. 11-10
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VVP Fig. 11-17
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activity vs. pH for lysozyme
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Explain the pH optimum.
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activity vs. pH for lysozyme
D52 must be deprotonated to stabilize oxonium ion
E35 acts as GAC catalyst in RDS oxonium ion
formation
D52
COOH lt COO-
E35
COOH gt COO-
E35
D52
COOH lt COO-
COOH gt COO-
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VVP Fig. 11-27
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