John A.G. Briggs

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John A.G. Briggs Retrovirus structure and
assembly
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The structural genomics of HIV
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Gag
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Immature virion
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Immature HIV
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p17
p24
p7
p2
p1
Gag cleavage can be inhibited
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maturation
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MMTV
Immature virion
HIV
2 pathways of Retrovirus formation.
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Immature virion
Internal scaffold domain
Cytoplasmic retention motif
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MMTV
Immature virion
HIV
Envelopment?
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cytosol
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cytosol
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maturation
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Finch/Sundquist lab
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Core formed from 1200 capsid proteins Arranged
in 96Å hexagonal lattice
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maturation
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In-vitro assembled
Immature HIV-1
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If it is made from 80A hexagons, with six
proteins per hexagon then 3400 200 gag proteins
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Scanning Transmission Electron Microscopy
Mean mass 165 29 MDa 3800 700 gag proteins
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145nm immature virion formed from 5000 Gag
proteins Arranged in 80 Å hexagonal lattice
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Immature virion Tighter hexagonal lattice with 8
nm unit cell. 5000 gag proteins form a complete
shell for a 145 nm virus
Mature virion Looser hexagonal lattice with 9.6
nm unit cell. 1200 CA proteins form the conical
core, leaving a large number elsewhere
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Virology University of Heidelberg University of
Oxford Cornell University Hans-Georg
Kraüsslich Barbara Müller Marko Lampe Bärbel
Glass Marc Johnson Volker Vogt Quentin Sattentau
Electron Microscopy University of
Oxford Max-Planck Institute for
Biochemistry Stephen Fuller Kay
Grünewald Friedrich Förster Thomas Wilk Giulia
Zanetti