Lab Report Review

1
Lab Report Review

• Title
• Hypothesis
• Materials
• Procedure
• Data Table
• Conclusion

2
DNA and RNA Staining Lab

• Purpose To prepare a histochemical slide of
  onion root tip during mitosis, in order to
  observe and identify nucleic acids, through
  staining.
• Hypothesis Make a hypothesis about how staining
  will aid in identifying the different nucleic
  acids in plant cells.
• Safety procedures Dont get the stain on your
  skin or clothes.

3
Lab Procedures

• Place slide on a paper towel and put one to two
  drops of stain on the center
• Place one onion root tip on the stain and gently
  mash it with the wood stick.
• Let stand for 10 minutes
• Place cover slip on the slide and use the paper
  towel to push down on the cover slip slightly
• Observe under low and high power.
• Record data in table

4
Copy table onto your lab paperData Table
Structure Stained Color (experimental)
DNA
RNA
Nucleus
Nucleolus observed__
Cytoplasm
Stained Color (expected)





Blue/purple
Pink/red
Blue/purple
Pink/red
5
Analysis

• Draw and label a plant cell from your
  preparation. Include all visible structures and
  color it the way it stained.
• How many nucleoli (on average) did you see in the
  cells?
• What are the structural differences between DNA
  and RNA that may have caused them to stain
  differently?
• Explain why certain cellular structures stained
  the same color (hint think about what they do or
  contain)