Effectiveness of Lookingglass Hatchery UV Disinfection Water System

1
Effectiveness of Lookingglass Hatchery UV
Disinfection Water System

• Sam Onjukka, ODFW Fish Health Services
• And
• Diane Deal, Assistant Mgr. Lookingglass Hatchery

2
Lookingglass Fish HatcheryCame on-line in 1982

• 1999 a study conducted by Dr. Warren Groberg, Sam
  Onjukka others, recommended developing a
  pathogen free water supply for LGH for disease
  management

3
Ultraviolet System UV SystemWent on-line in
March, 2006

• Drum Filter
• UV Shell/UV lights
• Inlet/Outlet Weir Boxes
• Gravity flow Very simple and effective system
• Original Cost, 285,000 UV System Building

4
Part 1 -Maintenance

• Weekly Check spray bar nozzles
• Hourly Lube drum filter, two bogie wheels and
  zirks
• Monthly Clean lamps, spray bar filter every
  other month
• Yearly all other service once a year which
  includes changing bulbs, cleaning crystals,
  pressure washing, filter cleaning, etc.
• Maintain a Maintenance Log

5
Part 1 Related ExpensesJuly, 2007 Prices

• Filter Panels 186.00 each
• Screen Patching Plugs 1.28 each
• O-Rings 4.00 a pack of 10
• Nozzles 4.50 each
• UV Lamps 100.00 each
• UV Crystals 100.00 each
• Complete back-up sets are kept on station

6
Maintenance Log
7
Back-Up SystemJuly 2007 Quote

• Cost 14,323.18
• Additional 14 lamps with Quartz sleeves insert
  kit, and Control panel additional dosage or
  back-up in case of lamp/panel failure

8
Natural Rearing can now be accomplished because
of the UV Treated water

• All of Lookingglass Hatcherys program is being
  reared at the facility
• Frees up facilities that were rearing our fry,
  for other programs
• Cost effective chillers are shut down as soon
  as we can put eggs on UV treated creek water
  Well water supplementation becomes lt 25 and more
  water is available for de-icing the Intake
• Daily electrical cost, UV 2.86/day, Chiller
  8.03 day, Well 215.37/day per 2007 rates

9
Part 2 Fish Health Effectiveness of
Lookingglass Hatcherys UV disinfection water
system

• Less Pathogen load
• Better water quality Better Fish Health

10
Effectiveness of Lookingglass Hatchery UV
Disinfection Water System Bacterial counts in
Pre and Post UV treated Water

• Sam Onjukka
• ODFW Fish Health Services
• Eastern Oregon University, La Grande, Oregon
• Diane Deal
• ODFW - Lookingglass Hatchery

11
Fish Health Recommendations to Reduce the
Incidence and Severity of Disease (2002)
12
Lookingglass Hatchery UV System Flow vs. Dose
Relationship
13
Effectiveness of the UV SystemMeasured by a
reduction in total bacterial counts in post UV
treated water as compared to raw Lookingglass
Creek water pre UV treated water
14
Bacterial Testing Procedure

• Water samples collected in sterile containers,
  kept dark on ice.
• Each sample diluted 10 fold in sterile 0.5 NaCl
• 0.1 ml of each sample and dilution plated onto
  Tryptone Yeast Extract agar (TYE-S) agar plates
  in replicate and spread with a flame sterilized
  glass hockey stick.
• Plates incubated at 18 C for 7 days.
• Bacterial colonies counted and original
  concentration in original sample calculated
• (colony forming units per ml cfu/ml).

15
(No Transcript)
16
(No Transcript)
17
(No Transcript)
18
Bacterial Counts in Pre and Post-UV Treated Water
at Lookingglass Hatchery in 2006-2007
48 reduction
96 and 95 reduction
Post UV sampled at outlet weir box
61 reduction
33 reduction
57 reduction
No reliable data
No reliable data
a plating at hatchery b plating at laboratory
19
(No Transcript)
20
Bacterial Counts in Lookingglass Creek Water
Above Lookingglass Hatchery Intake
21
Bacterial Counts in Pre and Post-UV Treated Water
at Lookingglass Hatchery in 2007-2008
100 reduction
99.9 reduction
99.1 reduction
92.6 reduction
99.8 reduction
160 gpm 335,663 mw
250 gpm 300,000 mw
258 gpm 300,000 mw
258 gpm 300,000 mw
680 gpm 98,000 mw
22
Summary

• Total bacterial counts were useful in measuring
  the effectiveness of the UV system once methods
  and sample locations were worked out.
• The UV system appears to be effective at reducing
  99 or more of the total culturable bacteria with
  lower flow rates (up to 250-300 GPM) but there
  is concern as flows increase. February 2008
  results showed 92.7 reduction with 680 GPM.
• The apparent slightly reduced effectiveness of
  the UV system at higher flow rates raises some
  concern for the protection of production fish
  from pathogens shed in the water supply by passed
  adults or naturally reared fish.
• UV treated water is only for early rearing. Once
  fish are ponded in outside raceways (April or
  early May) they have 10 months or more of rearing
  on raw Lookingglass Creek water.
• Monitoring the UV system fish health over time
  will make it clearer just how effective the
  system will be.

23
Any advantage we give the fish, from egg to
adults this is the result, Yes!
24
Thanks to everyone who has made this program a
success