Chapter 5 Nutrition, Culture, and Metabolism of Microorganisms

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Chapter 5 Nutrition, Culture, and Metabolism of
Microorganisms
Oxidation-Reduction Reactions
Nutrition and Culture
Energetics and Enzymes
Essentials of Catabolism
Essentials of Anabolism
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FINAL ROUND
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Topic 1 Nutrition and Culture 100 Question

• Question Siderophores help transfer which of the
  following macronutrients across the cell
  membrane?
• a. nitrogen
• b. phosphorous
• c. iron
• d. potassium

ANSWER
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Topic 1 Nutrition and Culture 100 Answer

• Question Siderophores help transfer which of the
  following macronutrients across the cell
  membrane?
• a. nitrogen
• b. phosphorous
• c. iron
• d. potassium

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Topic 1 Nutrition and Culture 200 Question

• Question Which of the following macronutrients
  makes up both amino acids and nucleotides?
• a. nitrogen
• b. phosphorous
• c. iron
• d. potassium

ANSWER
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Topic 1 Nutrition and Culture 200 Answer

• Question Which of the following macronutrients
  makes up both amino acids and nucleotides?
• a. nitrogen
• b. phosphorous
• c. iron
• d. potassium

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Topic 1 Nutrition and Culture 300 Question

• Question Which of the following is considered a
  growth factor?
• a. iron
• b. vitamins
• c. hydroxamic Acid
• d. potassium

ANSWER
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Topic 1 Nutrition and Culture 300 Answer

• Question Which of the following is considered a
  growth factor?
• a. iron
• b. vitamins
• c. hydroxamic Acid
• d. potassium

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Topic 1 Nutrition and Culture 400 Question

• Question In which type of media would an
  investigator add specific factors or nutrients
  that would allow certain organisms to grow that
  would not normally grow?
• a. chemically defined
• b. dIfferential
• c. selective
• d. enrichment

ANSWER
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Topic 1 Nutrition and Culture 400 Answer

• Question In which type of media would an
  investigator add specific factors or nutrients
  that would allow certain organisms to grow that
  would not normally grow?
• a. chemically defined
• b. differential
• c. selective
• d. enrichment

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Topic 1 Nutrition and Culture 500 Question

• Question Which type of media allows the bacteria
  to become visible and form colonies?
• a. liquid culture
• b. solid culture
• c. chemically defined media
• d. chemically complex media

ANSWER
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Topic 1 Nutrition and Culture 500 Answer

• Question Which type of media allows the bacteria
  to become visible and form colonies?
• a. liquid culture
• b. solid culture
• c. chemically defined media
• d. chemically complex media

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Topic 2 Energetics and Enzymes100 Question

• Question If ?G0' lt 0, then _______________.
• a. the reaction is exergonic and can occur
  spontaneously
• b. the reaction is endergonic and can occur
  spontaneously
• c. the reaction is exergonic and cannot occur
  spontaneously
• d. the reaction is endergonic and cannot occur
  spontaneously

ANSWER
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Topic 2 Energetics and Enzymes100 Answer

• Question If ?G0' lt 0, then _______________.
• a. the reaction is exergonic and can occur
  spontaneously
• b. the reaction is endergonic and can occur
  spontaneously
• c. the reaction is exergonic and cannot occur
  spontaneously
• d. the reaction is endergonic and cannot occur
  spontaneously

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Topic 2 Energetics and Enzymes200 Question

• Question Which of the following are
  characteristics of enzymes?
• a. they are consumed by the enzyme-
  mediated reaction
• b. they are not altered by the enzyme-
  mediated reaction
• c. they raise activation energy
• d. all of the above

ANSWER
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Topic 2 Energetics and Enzymes200 Answer

• Question Which of the following are
  characteristics of enzymes?
• a. they are consumed by the enzyme-
  mediated reaction
• b. they are not altered by the enzyme-
  mediated reaction
• c. they raise activation energy
• d. all of the above

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Topic 2 Energetics and Enzymes300 Question

• Question Which of the following terms relate to
  enzymes?
• a. proteins
• b. active site
• c. catalysts
• d. all of the above

ANSWER
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Topic 2 Energetics and Enzymes300 Answer

• Question Which of the following terms relate to
  enzymes?
• a. proteins
• b. active site
• c. catalysts
• d. all of the above

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Topic 2 Energetics and Enzymes400 Question

• Question How do you calculate the ?G0' of a
  reaction?
• a. add the Gf0 of all of the products and then
  subtract the Gf0 of all of the reactants
• b. add the Gf0 of all of the reactants and then
  add the Gf0 of all of the products
• c. subtract the Gf0 of any products and add
  the Gf0 of the reactants
• d. subtract the Gf0 of any reactants and then
  add the Gf0 of the products

ANSWER
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Topic 2 Energetics and Enzymes400 Answer

• Question How do you calculate the ?G0' of a
  reaction?
• a. add the Gf0 of all of the products and then
  subtract the Gf0 of all of the reactants
• b. add the Gf0 of all of the reactants and then
  add the Gf0 of all of the products
• c. subtract the Gf0 of any products and add
  the Gf0 of the reactants
• d. subtract the Gf0 of any reactants and then
  add the Gf0 of the products

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Topic 2 Energetics and Enzymes500 Question

• Question Which of the following molecules do
  enzymes associate with in catalyzing a reaction?
• a. prosthetic groups
• b. coenzymes
• c. both prosthetic groups and coenzymes
• d. neither prosthetic groups nor coenzymes

ANSWER
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Topic 2 Energetics and Enzymes500 Answer

• Question Which of the following molecules do
  enzymes associate with in catalyzing a reaction?
• a. prosthetic groups
• b. coenzymes
• c. both prosthetic groups and coenzymes
• d. neither prosthetic groups nor coenzymes

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Topic 3 Oxidation-Reduction Reactions 100
Question

• Question If organism A used H2S as an electron
  donor and Fe3 as an electron acceptor (at pH of
  7) and organism B used methanol as an electron
  donor and O2 as an electron acceptor, which
  organism can probably produce most energy.
• a. organism A
• b. organism B
• c. both produce probably the same amount
  of energy

ANSWER
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Topic 3 Oxidation-Reduction Reactions 100
Answer

• Question If organism A used H2S as an electron
  donor and Fe3 as an electron acceptor (at pH of
  7) and organism B used methanol as an electron
  donor and O2 as an electron acceptor, which
  organism can probably produce most energy.
• a. organism A
• b. organism B
• c. both produce probably the same amount
  of energy

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Topic 3 Oxidation-Reduction Reactions 200
Question

• Question Which of the following statements
  concerning NADH is true compared to other
  molecules in the electron transport chain?
• a. it has the most negative E0 and the greatest
  capacity to accept electrons
• b. it has the most negative E0 and the greatest
  capacity to donate electrons
• c. it has the most positive E0 and the greatest
  capacity to accept electrons
• d. it has the most positive E0 and the greatest
  capacity to donate electrons

ANSWER
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Topic 3 Oxidation-Reduction Reactions 200
Answer
Question Which of the following statements
concerning NADH is true compared to other
molecules in the electron transport chain? a. it
has the most negative E0 and the greatest
capacity to accept electrons b. it has the most
negative E0 and the greatest capacity to donate
electrons c. it has the most positive E0 and the
greatest capacity to accept electrons d. it has
the most positive E0 and the greatest capacity
to donate electrons
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Topic 3 Oxidation-Reduction Reactions 300
Question

• Question Which of these groups are carried by
  NADH and FADH2 and will ultimately lead to the
  production of ATP in the electron transport
  chain?
• a. electrons
• b. hydrogen ions
• c. acetyl CoA
• d. phosphorous
• e. a and b

ANSWER
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Topic 3 Oxidation-Reduction Reactions 300
Answer

• Question Which of these groups are carried by
  NADH and FADH2 and will ultimately lead to the
  production of ATP in the electron transport
  chain?
• a. electrons
• b. hydrogen ions
• c. acetyl CoA
• d. phosphorous
• e. a and b

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Topic 3 Oxidation-Reduction Reactions 400
Question

• Question Which of the following molecules could
  serve as an electron donor in the electron
  transport chain?
• a. FAD
• b. NADH
• c. NAD
• d. NADPH

ANSWER
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Topic 3 Oxidation-Reduction Reactions 400
Answer

• Question Which of the following molecules could
  serve as an electron donor in the electron
  transport chain?
• a. FAD
• b. NADH
• c. NAD
• d. NADPH

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Topic 3 Oxidation-Reduction Reactions 500
Question

• Question During which of the following processes
  does NAD get regenerated so that it can be used
  again in glycolysis?
• a. Krebs cycle
• b. electron transport chain
• c. in yeast, conversion of pyruvic acid to
  ethanol and carbon dioxide
• d. a and c
• e. b and c

ANSWER
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Topic 3 Oxidation-Reduction Reactions 500
Answer

• Question During which of the following processes
  does NAD get regenerated so that it can be used
  again in glycolysis?
• a. Krebs cycle
• b. electron transport chain
• c. in yeast, conversion of pyruvic acid to
  ethanol and carbon dioxide
• d. a and c
• e. b and c

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Topic 4 Prokaryotic Structures 100 Question

• Question Which of the following reactions in
  glycolysis involve substrate-level
  phosphorylation?
• a. phosphoenolpyruvate ? pyruvate
• b. glucose? Glucose-6-phosphate
• c. 1,3 bisphosphoglycerate ? 3- phosphoglycerat
  e
• d. a and b
• e. a and c

ANSWER
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Topic 4 Essentials of Catabolism 100 Answer

• Question Which of the following reactions in
  glycolysis involve substrate-level
  phosphorylation?
• a. phosphoenolpyruvate ? pyruvate
• b. glucose? Glucose-6-phosphate
• c. 1,3 bisphosphoglycerate ? 3- phosphoglycerate
  
• d. a and b
• e. a and c

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Topic 4 Essentials of Catabolism 200 Question

• Question What is the purpose of the Krebs cycle?
  
• a. to make ATP
• b. to completely oxidize the pyruvate that
  comes from glycolysis
• c. to form molecules that will shuttle
  electrons to the electron transport chain
• d. to make some intermediates that can be used
  during biosynthesic reactions
• e. all of the above

ANSWER
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Topic 4 Essentials of Catabolism 200 Answer

• Question What is the purpose of the Krebs cycle?
  
• a. to make ATP
• b. to completely oxidize the pyruvate that
  comes from glycolysis
• c. to form molecules that will shuttle
  electrons to the electron transport chain
• d. to make some intermediates that can be used
  during biosynthesic reactions
• e. all of the above

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Topic 4 Essentials of Catabolism 300 Question

• Question During which stage of cellular
  respiration are both NADH and FADH2 produced?
• a. glycolysis
• b. conversion of pyruvate to acetyl CoA
• c. Krebs cycle
• d. electron transport chain

ANSWER
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Topic 4 Essentials of Catabolism 300 Answer

• Question During which stage of cellular
  respiration are both NADH and FADH2 produced?
• a. glycolysis
• b. conversion of pyruvate to acetyl CoA
• c. Krebs cycle
• d. electron transport chain

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Topic 4 Essentials of Catabolism 400 Question

• Question What is the correct order of transfer
  of electrons in the electron transport chain?
• a. oxygen, cytA, cytC, Q, FMN, NADH
• b. FMN, NADH, Q, cytC, cytA, oxygen
• c. NADH, FMN, Q, cytC, cytA, oxygen
• d. cytA, oxygen, cytC, NADH, FMN, Q

ANSWER
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Topic 4 Essentials of Catabolism 400 Answer

• Question What is the correct order of transfer
  of electrons in the electron transport chain?
• a. oxygen, cytA, cytC, Q, FMN, NADH
• b. FMN, NADH, Q, cytC, cytA, oxygen
• c. NADH, FMN, Q, cytC, cytA, oxygen
• d. cytA, oxygen, cytC, NADH, FMN, Q

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Topic 4 Essentials of Catabolism 500 Question

• Question Where are the different complexes of
  the electron transport chain located in the
  mitochondria?
• a. outer membrane
• b. inner mitochondrial membrane
• c. inner membrane space
• d. matrix

ANSWER
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Topic 4 Essentials of Catabolism 500 Answer

• Question Where are the different complexes of
  the electron transport chain located in the
  mitochondria?
• a. outer membrane
• b. inner mitochondrial membrane
• c. inner membrane space
• d. matrix

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Topic 5 Essentials of Anabolism100 Question

• Question In prokaryotics, polysaccharides are
  synthesized from either _______________.
• a. UDPG or NADPH
• b. NAM or ADPG
• c. UDPG or ADPG
• d. UDPG or NAG

ANSWER
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Topic 5 Essentials of Anabolism 100 Answer

• Question In prokaryotics, polysaccharides are
  synthesized from either _______________.
• a. UDPG or NADPH
• b. NAM or ADPG
• c. UDPG or ADPG
• d. UDPG or NAG

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Topic 5 Essentials of Anabolism 200 Question

• Question The process of synthesizing glucose in
  a cell is called _______________.
• a. anaerobic respiration
• b. glutamine synthetase
• c. gluconeogenesis
• d. fermentation
• e. anabolism

ANSWER
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Topic 5 Essentials of Anabolism 200 Answer

• Question The process of synthesizing glucose in
  a cell is called _______________.
• a. anaerobic respiration
• b. glutamine synthetase
• c. gluconeogenesis
• d. fermentation
• e. anabolism

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Topic 5 Essentials of Anabolism 300 Question

• Question The enzyme ribonucleotide reductase is
  used in the synthesis of _______________.
• a. DNA
• b. RNA
• c. amino acids
• d. lipids

ANSWER
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Topic 5 Essentials of Anabolism 300 Answer

• Question The enzyme ribonucleotide reductase is
  used in the synthesis of _______________.
• a. DNA
• b. RNA
• c. amino acids
• d. lipids

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Topic 5 Essentials of Anabolism 400 Question

• Question Which of the following is used in the
  biosynthesis of amino acids and is produced in
  the Krebs cycle?
• a. glutamate
• b. glutamine
• c. a-ketoglutarate
• d. ammonia

ANSWER
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Topic 5 Essentials of Anabolism 400 Answer

• Question Which of the following is used in the
  biosynthesis of amino acids and is produced in
  the Krebs cycle?
• a. glutamate
• b. glutamine
• c. a-ketoglutarate
• d. ammonia

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Topic 5 Essentials of Anabolism 500 Question

• Question Inosinic acid is the precursor of
  _______________.
• a. adenine and guanine
• b. adenine and cytosine
• c. thymine and cytosine
• d. guanine and thymine

ANSWER
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Topic 5 Essentials of Anabolism 500 Answer

• Question Inosinic acid is the precursor of
  _______________.
• a. adenine and guanine
• b. adenine and cytosine
• c. thymine and cytosine
• d. guanine and thymine

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FINAL ROUND Question

• Question If something was not working properly
  in the cell and pyruvate could not be converted
  into acetyl CoA, then what would be the total
  production of ATP at the end of all the stages in
  cellular respiration that could occur?
• a. 2
• b. 8
• c. 14
• d. 36

ANSWER
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FINAL ROUND Answer

• Question If something was not working properly
  in the cell and pyruvate could not be converted
  into acetyl CoA, then what would be the total
  production of ATP at the end of all the stages in
  cellular respiration that could occur?
• a. 2
• b. 8
• c. 14
• d. 36

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