General Aspects of Metabolism

1
Carbohydrates Lipids Protein
1
2
2
Glucose
Glycolysis
Pyruvate
Bridging Rx.
AcetylCoA
NADH FADH2
C6
C4
Krebs Cycle
OP
C5
C4
ATP
3
Oxidative Phosphorylation(Respiratory Chain)
3
NADH, H 1/2 O2 3ADP, Pi
NAD H2O 3ATP
Electron transport
4
Oxidative Phosphorylation
4
NADH, H 1/2 O2 3ADP, Pi
NAD H2O 3ATP
5
Oxidative Phosphorylation Complexes In
Mitochondrial Inner Membrane
5
6
6
Electron Transport Complexes
NADH-CoQ Reductase 880k
CoQ Cytochrome C Reductase 250k
Cytochrome Oxidase 160k
7
Oxidative Phosphorylation Proton
Gradient
7
H
8
8
NADH Q ? NAD QH2
C(red) O ? H2O C(ox)
QH2 C(ox) ? Q C(red)
9
Chemiosmotic Theory Proton Gradient
9
DpH -1.4
10
Proton Gradient Energy Gradient
10
11
H
ATP synthase Fo
F1
ADP Pi
11
12
H
H
12
II
III
I
FADH2 gt FAD
only 2 ATP gradient produced
13
OP UncouplersTransport H across membrane
without generating ATP
13
1. 2,4 - dinitrophenol weak NP acid
2. UCP (uncoupler Protein) passive H
transport
14
14
H
15
Sources of NADH (FADH2)
15
Glucose Fatty Acid amino
acid glycolysis b oxidation oxidative

deamination Bridging Rx
Bridging Rx
Krebs Cycle