Project Abiosn'032192

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Project A-bios-n.032192

• Report of the first research period
• P.Valenti, Colaizzi P. Pinzari F.
• Ist. Patologia del Libro,Ministry Cutural
  Heritage
• Reverberi M., Zjalic S. C.Fanelli
• Università di Roma La Sapienza

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• Participants
• R.G.I, Genoa Italy- Lead participant WP1-WP3
• AMOS, Brussels, Belgium- Lead participant WP2
• NILU, Oslo, Norway- Lead participant WP4
• MNK, Krakow, Poland- Lead participant WP5
• UNIRO, Roma, Italy- Lead participant WP6-WP8 and
  coordinator
• ISNOVA, Turin, Italy- Lead participant WP7
• MYCOTEAM, Oslo, Norway

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A-BIOS device

• Filtration of air and sterilization on UVC lamps
• Laboratory field tests carried out with the
  first prototype
• A second prototype designed upon the requests of
  contractors has been fabricated

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LABORATORY FIELD TESTS WITH THE A-BIOS PROTOTYPE

• LABORATORY OF PLANT PATHOLOGY AND MYCOLOGY,
  UNIVERSITA LA SAPIENZA
• LABORATORY OF BIOLOGY
• ISTITUTO CENTRALE DI PATOLOGIA DEL LIBRO

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LABORATORY TESTSFirst group of experiments

• Effect of A-BIOS first prototype on conidia of
  Aspergillus terreus and Penicillium expansum
• Conidia were disseminated in a small room (8m3)
  previously sterilized by UVC
• Conidia were collected on inlet and outlet
  filters of the prototype after A-BIOS treatment
  for different times

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Viability of Penicillium chrysogenum conidia
treated by A-BIOS (CFU)
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Viability of Aspergillus terreus conidia treated
by A-BIOS (CFU)
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Diagram showing the decreased viability of fungal
conidia after treatment by A-BIOS
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LABORATORY TESTSSecond group of experiments

• Monitor of mycoflora before and after treatment
  by A-BIOS in a closed small room (10m3) (severely
  contaminated by fungi on the walls)

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Inhibition of conidia germination isolated from
air in room treated by A-BIOS (after 2 days of
incubation)
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Inhibition of conidia germination isolated from
air in room treated by A-BIOS (after 6 days of
incubation)
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Diagram showing the decreased viability of fungal
conidia after treatment by A-BIOS
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Conclusions - I

• Treatment by A-BIOS severely lowered viability of
  conidia present in air
• The germination time was prolonged (after 6 days
  of incubation is present an higher number of
  colonies than after 2 days in treated samples)
• These data are in accordance with other studies
  (Sanchez et al., 2007)

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IN FIELD TESTSThird group of experiments

• Effect of short exposure time (0-30 min)
  treatment with A-BIOS on mycoflora present in
  David Lubin Memorial Library of FAO in Rome
• Huge space (320 m3) with a number of shelve rows
  partially isolated each from others
• Air conditioned (23C) room, no control of
  humidity
• In some shelves are present books contaminated by
  fungi

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Room with not contaminated books
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Room with contaminated books
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Conclusions - II

• A-BIOS sterilizes air in short time in a small
  space, whereas, with a lower efficiency in larger
  and air conditioned room

• Longer time of exposition to UVC would be
  necessary to obtain a marked inhibition of fungal
  spore viability

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IN FIELD TESTSFourth group of experiments

• Effect of long exposure time treatment (0-6h)
  with A-BIOS on mycoflora present in David Lubin
  Memorial Library of FAO in Rome
• Same conditions as in third group of experiments
  limited to the room with contaminated books

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Room with contaminated books
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Statistical analysis
Comparison among sampling times. Tukey (HSD)
test.
Comparison among sampling times. Fisher (LSD)
test
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Conclusions - III

• A-BIOS device significantly (plt0.0001) decreases
  the amount of viable conidia in large, air
  conditioned, contaminated rooms after 3h of
  treatment
• Treatment by A-BIOS lowered viability of all
  conidia present in air
• A-BIOS represents a simple but useful tool to
  limit the contamination of papery materials
  stored in library