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Portable Water Scanner PWS

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Two DNA strands: substrate and the catalytic DNAzyme. RNA present in substrate ... Resolve complications with A/D converter using PIC controller ... – PowerPoint PPT presentation

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Title: Portable Water Scanner PWS


1
Portable Water Scanner (PWS)
  • ECE 445 - Senior Design
  • Project 17
  • Members Ogden Dlima Robin O
  • April 28, 2005

2
Overview
3
Introduction
  • Water Contamination is a big issue all around the
    world
  • More than 2,500,000 die each year from unsanitary
    water conditions
  • Water testing is very expensive and time
    consuming
  • Sample is taken from water source to the
    laboratory
  • Present water testers only check for generalized
    contamination
  • Unable to name contaminant and give concentration
  • If contaminated, water is treated using a
    generalized purifier
  • Unable to predict reaction with unknown
    contaminants

4
Initial Design Idea
  • PWS will check for specific contaminant and
    concentration in ppb
  • Will be able to treat only for the specific
    contaminant
  • Will be able to treat based on the observed
    concentration
  • Takes testing from the laboratory directly to the
    water source
  • Quick, inexpensive and accurate results
  • Portable device will be able to withstand all
    weather conditions
  • Platform for detection of all types of
    contaminants
  • Idea could be extended to other types of liquids
  • Choose LEAD as the contaminant for our design
  • Major issue with current drinking water sources

5
The Contaminant Lead
  • Highly toxic to humans, especially young children
  • Nervous system damage can lead to learning
    disabilities, coma, and death
  • Causes anemia, kidney failure and hypertension
  • Reproductive system effects include stillbirths,
    miscarriages, male infertility and neurological
    impairment in the fetus
  • Heavy metal, can be detected using a lead sensor
  • Does not exist freely in nature, only as Pb2
  • EPA regulation gt 15 ppb
  • Water is contaminated and unsafe to drink
  • If lt 15 ppb, passes through body without any
    problems

6
Product Features
  • Identifies the presence of lead
  • Quantifies results to ppb and displays on LCD
  • Offers portability and long life via two 9-V
    alkaline batteries
  • Offers small and compact design for on-site
    testing
  • Offers waterproof and robust casing to prevent
    damage to inner circuits
  • Provides slot for water sample and air-tight
    capping
  • Box is completely sealed off to prevent any light
    from entering in

7
Product Benefits
  • Educates users of the presence of lead in water
  • Reduces health risks due to consumption of
    contaminated water
  • User-friendly device
  • Universal ability to check for water
    contamination at the source
  • Quick treatment of water based on contaminants
    detected
  • Portable device due to compact design and long
    battery life
  • Base platform for detection of a whole range of
    contaminants

8
DNA based Bio-Sensor for Lead
  • Initial idea was to design our own lead sensor
  • Prof. Yi Lu and the Lu Group develop DNA based
    biosensor to detect divalent lead
  • Two DNA strands substrate and the catalytic
    DNAzyme
  • RNA present in substrate
  • DNA strands are broken in presence of Pb2 and
    there is fluorescence glowing state
  • DNA strands can be engineered to be specifically
    sensitive to any such metal ions
  • Fluorescent intensity proportional to lead
    concentration

9
Graphs
10
Original Design
11
Initial Block Diagram
12
Initial Schematic for PWS
13
Revised Project Build
14
Revised Block Diagram
15
Light Detector Circuit
16
Software Flowchart
  • Pseudo Code
  • Initialization/Reset State
  • Initialize and begin LCD Display start sequence
  • Define default calibration value in one 8-bit
    word
  • Clear old 8-bit ppb value
  • Go to First Wait State
  • Scan State
  • Begin voltage regulation sequence
  • Begin ADC sequence
  • Read 8-bit output of ADC
  • Determine 8-bit subtraction of calibration
    value from ADC output
  • Compute and convert result into three numerical
    values for ppb readout
  • Go to Display State
  • Display State
  • Enable three corresponding characters for ppb
    value
  • Display ppb
  • Go to Second Wait State
  • First Wait State

17
5v Regulated DC Power Supply
  • Two 9v Alkaline Batteries in series as input
  • 5v regulated DC output
  • LM7805C 3 Terminal Positive voltage regulator
  • SPST On/Off Switch to prevent draining the
    battery
  • LED lights up when power is supplied to the
    circuit

18
18v Battery Monitor
  • R3(potentiometer) controls the trip-point of the
    circuit
  • When the voltage falls below the trip-point, the
    LED lights
  • The trip-point for the 18v battery is set at 8v
  • Below 8v, the 3 terminal positive voltage
    regulator will not function properly

19
Over-voltage Protection Circuit
  • Protects the micro-controller and LCD from excess
    DC regulated voltage
  • Can switch off the output should the input
    voltage raise above 5.6v
  • Max. current circuit can handle is 1 Amp
  • 1N4734 - 5.6v Breakdown zener
  • BC108 -- Low power NPN Transistor
  • BFY51 NPN transistor capable of switching

20
Current Boost Circuit
21
Initial Drawing of Portable Device
22
Portable Water-proof Box
23
Functional Tests Procedures
24
Testing Procedures
  • Tested output voltage of initial
    photocell/photoresistors
  • Results showed not enough sensitivity to relevant
    wavelength range (520 565 nm)
  • Tested output voltage of photodiode to be sent to
    A/D converter
  • With one LED, found a less consistent and linear
    relationship between brightness and photodiode
    output voltage
  • With two LEDs, found a more consistent and
    linear relationship between brightness and
    photodiode output voltage
  • Replaced testing of DNA with Fluorescein for
    light detection circuit
  • DNA is too expensive and time consuming to
    produce
  • Tested output of power supply using oscilloscope
  • Showed minimal variations from expected 5 volt
    output (30mV)

25
Test Results for Light Detection
26
LCD Demonstration
27
Power Supply Output
  • X-axis 100µs/div
  • Y-axis 1.00v/div
  • Output Voltage 5 volts
  • X-axis 2.00µs/div
  • Y-axis 20mv/div
  • Ripple 30mv

28
Successes and Challenges
  • Succeeded with design of portable power supply
  • Succeeded with design of the light detection
    circuit
  • Succeeded with finding linear relationships
    between light intensity and photodiode output
    voltage
  • But could not detect enough light from
    Fluorescein to produce noticeable voltage change
  • Succeeded with LCD controls using PIC controller
  • But could not obtain input from A/D converter
  • Did not succeed with A/D converter

29
Engineering Solutions
  • To overcome Shortcomings in design
  • Use a photodiode more sensitive to needed
    wavelength (520 565nm)
  • Resolve complications with A/D converter using
    PIC controller
  • Apply linear relationship determined by earlier
    tests using PIC
  • Calculate and convert lead content in terms of
    ppb to LCD display

30
Questions
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