Chem Club Dinner

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Chem Club Dinner at Bob's in Fairhaven F 530 pm
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DNA Footprinting
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Figure 31-10 The sense (nontemplate) strand
sequences of selected E. coli promoters.
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Watson Figure 12-6
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Watson Figure 12-7
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?
Protein-NA interactions DBS DNA binding
site RBS RNA binding site HBS Hybrid binding
site
?
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Figure 31-16 An electron micrograph of three
contiguous ribosomal genes from oocytes of the
salamander Pleurodeles waltl undergoing
transcription.
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Backtracking for editing
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Watson Figure 12-9
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Watson Fig. 12-11 Rho
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?-Independent termination
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Post-transcriptional Processing
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Eukaryotic RNA gets a 5 cap...
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and a polyA tail
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U1 bound to splice site
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http//www.whfreeman.com/lodish/con_index.htm?99an
m
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?-Tropomyosin alternative splicing
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MVA Fig. 28.30
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RNA SPLICING!!!
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Figure 31-46 An electron micrograph and its
interpretive drawing of a hybrid between the
antisense strand of the chicken ovalbumin gene
and its corresponding mRNA.
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Figure 31-47 The sequence of steps in the
production of mature eukaryotic mRNA as shown for
the chicken ovalbumin gene.
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Figure 31-48 The consensus sequence at the
exonintron junctions of vertebrate pre-mRNAs.
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Figure 31-49 The sequence of transesterification
reactions that splice together the exons of
eukaryotic pre-mRNAs.
1. The 2-OH group of a specific intron A
residue nucelophilically attacks the 5
-phosphoate at the 5- intron boundary
producing a 2,5-cyclic structure.
2. The liberated 3-OH group attacks the
5-phosphate of the 5-terminal residue of the
3exon, forming a 3,5-PD bond and displacing
the intron lariat.
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Figure 31-56 An electron micrograph of
spliceosomes in action.
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Figure 31-57 A schematic diagram of six
rearrangements that the spliceosome undergoes in
mediating the first transesterification reaction
in pre-mRNA splicing.
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Figure 31-61a The electron microscopy-based
structure of U1-snRNP at 10 Å resolution. (a) The
predicted secondary structure of U1-snRNA.
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Figure 31-62 The organization of the rat
a-tropomyosin gene and the seven alternative
splicing pathways that give rise to cell-specific
a-tropomyosin variants.
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Figure 31-64 The sequence of transesterification
reactions that occurs in trans-splicing.
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Figure 31-68 A model for RNA interference
(RNAi).
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Figure 31-69 A model for transitive RNAi.
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Figure 31-70 The posttranscriptional processing
of E. coli rRNA.
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Figure 31-72 The organization of the 45S primary
transcript of eukaryotic rRNA.
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Figure 31-73 A schematic diagram of the tRNA
cloverleaf secondary structure.
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Figure 31-74a The structure of the RNA of B.
subtilis RNase P. (a) Predicted secondary
structure with specificity domain drawn in
various colors and catalytic domain is black.
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Figure 31-75 The posttranscriptional processing
of yeast tRNATyr.
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Table 31-3 Amino Acid Sequences of Some Leader
Peptides in Operons Subject to Attentuation.
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Table 31-4 Types of Introns.
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from Genomes
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Introns in histone genes 0!!!
from Genomes
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Consensus sequence for vertebrate splicing
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1. The 2-OH group of a specific intron A
residue nucelophilically attacks the 5
-phosphoate at the 5- intron boundary
producing a 2,5-cyclic structure.
2. The liberated 3-OH group attacks the
5-phosphate of the 5-terminal residue of the
3exon, forming a 3,5-PD bond and displacing
the intron lariat.
VVP Fig. 25-20
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from Genomes
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Properties of eukaryotic snRNAs
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from Genomes
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MVA Fig. 28.31
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MVA Fig. 28.32
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MVA Fig. 28.33
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http//www.whfreeman.com/lodish/con_index.htm?99an
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MVA Fig. 28.34
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from Genomes
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1. The 3-OH group of a guanine nucleotide
attacks the introns 5-terminal phosphate
to form a new PD bond, displacing the 5-exon.
2. The new 3-)H group of the 5exon attacks the
5-terminal phosphate group of the 3-exon thus
splicing the 2 exons and displacing the intron.
3. The 3-OH group of the intron attacks the
phosphate 15 nts from its 5end thus cyclizing
the intron and displacing the 5terminal
fragment.
The internal H-bonds maintain the correct
conformation for precise excision of the intron.
VVP Fig. 25-23
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VVP Fig. 25-24 Catalytic Domain of Tetrahymena
Self-splicing Intron
Extensive H-bonding and Mg2 ions coordinated by
phosphate groups allow this structure to form an
enzyme likeinterior densely packed and solvent
inaccessible. The snRNAs probably evolved from a
structure like this, as did the rRNAs.
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from Genomes