Vanes Superfusion Cascade Bioassay

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Vanes Superfusion Cascade Bioassay
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The discovery of EDRF Nitric Oxide
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The discovery of EDHF Endothelium-Derived
Hyperpolarizing Factor
ACh
Control
5 mV
HB 3 µM
30 s
MB 3 µM
Smooth muscle cell membrane potential
Br J Pharmacol 951165, 1988
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KCa underlying the EDHF responses are located in
the endothelium
K released by these channels acts as a
diffusible hyperpolarizing factor
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by activating KCa2.3 KCa3.1
?Ca2 drives hyperpolarization
ACh
EC
SMC
Hyperpolarization drives ?Ca2
ACh
modified from Busse et al., 2002, TiPS 23374