L.V.Klimova

1
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SCENAR-THERAPY EFFECT ON THE STATE OF THE BLOOD
PLASMA REDOX BALANCE IN PATIENTS WITH ORGAN
ISCHEMIC INJURY
?.?. ??????? ?????????? ???????????????
??????????? ???????????, ?????? L.V.Klimova
Rostov State Medical University, Russia
2

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  ?????????????? ? ?????? (?????)
• ???????? ?????? ? ??????????????? ???????????
  ??????? ?? ??????? ????? ????????????
• ISCHEMIA
• is deficient or no blood supply to the organ
  (tissue)
• Ischemia and postischemic disorders will always
  be of current importance in medicine

3

• ??????
• ? ?????????????? ?? ????????, ?.?. ???????????
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  ??????, ??????????? ?? ????????? ????? ??
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• ? ?????, ?????????, ??????????? ?????????
  ??????????.

ISCHEMIA and accompanying hypoxia deficiency of
oxygen - is the trigger element for a number of
processes that inhibit the cell functionality at
first stages thus helping them to survive in
sudden oxygen shortcut, and subsequently cause
cell destruction
4

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• ?????????? ?????????????, ?.?. ?????????????
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  ????????? ?????????? ?? ??????????? ????
  ?????????????? ? ???????? ????????????????
  ????????? ????????.

Reperfusion - restoration of blood flow causes
reoxyganation, i.e. restoration of oxygen
supply to the tissue Cells can keep on
distructing because of oxidative paradox
pathological process when some portion of oxygen
doesnt undergo common way of restoration and
forms highly reactive toxic products
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• ??????????? ? ?????????????? ??????????? ????????
  
• ??????-?????? ?????????,
• ?.?. ??????????? ????????????-?????????????

Ischemic reperfusion injury changes the redox
balance (prooxidant antioxidant balance)
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• ? ?????????????
• ??????? ?????????????? ??????????? ??????????,
• ???????? ????? ????????? ? ????????? ????????
  ????? ?????????.

Prooxidants are activated oxygen metabolites,
active oxygen forms, free radicals of this
etiology
7

• ???????????? -
• ??? ??????????, ???????
• ??????????? ????????? ????????????????????
  ?????????
• ???? ?????????? ?,
• ? ?????????, ???????

Prooxidants are aggregations that activate free
radical oxidation of all boimolecules, in
particular lipids
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• ?????????? ????????? ??????? (???)
• ????????? ? ????????? ? ? ?????, ???????????
  ??????????? ????????? ?? ???? ?????????? ???????
  ????????? ???????, ?????? ?? ?? ??????-??????????
  ????????.

Lipid peroxidation (LOP) proceeds in the healthy
body maintaining the structural homeostasis by
renewing the lipids of cell membrane and
influences their physical-chemical properties
9

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• ?????????? ????????? ??? ???????? ??????????
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• ?????????? ????????? ????????.

BUT!!! High LOP activity is the result of
prooxidant content increase, while excessive LOP
products, especially final products, damage cell
membranes
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??????????????? ?????????? ?????? ???????????? ??
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???????

• ANTIOXIDANT
• is any substance which, when present at low
  concentrations relative to the substrate being
  oxidized, will inhibit or retard that oxidation
• Halliwell B., Gutteridge J.M.C. (1989)

Antioxidative aggregations differ in action
mechanisms, solubility, chemical nature
11

• ???????? (??)
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• ????????? ?????? ?? ? ?????? ????? ????????? ??
  ?? ????? ?? ???????????, ?????????? ????
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  ???? ??-?? ?? ??????????. ????? ????, ??????
  ?????????? ????????????? ???????? ??????????
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• ??? ???????? ? ???????? ??????? ????? ??????????
  ??? ????????? ?????????? ????????? ???????? ????
  ????????? ??? ???????? ??????????? ???????.

Catalase (CA) is an enzyme, which inactivates
hydrogen peroxide Essential part of the catalase
pool localizes in erythrocyte cytoplasm. In
extracellular water it soon becomes inactive. CA
increase in plasma can sign it has released from
the erythrocytes as a result of pathologically
high membrane permeability or even their
destruction. This index characterizes utilization
rate of hydrogen peroxide in the blood plasma.
Its decrease during the treatment can be
explained as result of decrease in generation of
active oxygen forms under the influence of the
given treatment .
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• ????????????? (??)
• - ?????????????? ?????, ???????? ????????
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• ??????????? ???? ???????? ?????????? ???????????,
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• ??? ???????? ???????? ??????????? ??????, ?.?.
  ?? ??????? ???????????? ?????????? ??????????????
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  ?????????.
• ????????? ? ?????? ?????? ????, ? ????????? ???
  ?????????? ??? ?????????? ??? ????? ?????? ??
  ????? ?????? ? 2-3 ????, ?? ??????, ?? 30-60
  ??????????????? ??? ?????????????? ???????,
  ???????????? ?? ?????????? ????????????????
  ?????????? ?????? ? ??????????? ??????????? ????
  ? ?????? ?? ??????????? ??????.

• Ceruloplasmin (CP)
• is a copper-containing protein, chelate compound,
  
• which connects transition metal ions that
  catalyze oxidation
• It is called iron regulator of the plasma as it
  causes decrease in concentration of free ferrous
  iron, which catalyzes hydroxyl radical formation.
  
• It belongs to proteins of acute phase and its
  activity increase in inflammation or after the
  injury - on one medical evidence by 2-3 times, on
  another by 30-60 - is considered as compensatory
  reaction directed to preserve plasma antioxidant
  potential and maintain optimal level of copper
  and zinc metabolism.

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Prooxidant - antioxidant disbalance, with
prooxidants prevailing and antioxidant systems
astrain, is also called oxidative stress.
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?????? ???????? Ischemia Hypoxia
?????????? ????????????? Reperfusion Reoxygenation
??????????? ?????? ? ?????? Cell and tissue
affection
????????? ??????-??????? ?????????????
?????? Redox disbalance Oxidative stress
15
? ???????? ?????? ???????????, ????? ?? ????????
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??????????? ?? ???? ?????????????? ?????? ?
?????? ??????? ????????.

• A large group of diseases where ischemia is one
  of the main pathogenic trigger, includes such
  severe diseases as acute renal failure caused by
  compression injury

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?????????????? ??????, ? ?????? Q-?????????? ???
? ??????? ?? ??? ??????? ??????-???????,
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???????? ?????? ??????????? ??????????? ?? ????
??????????? ??????? ?????????????? ???????.
A number of researches investigate oxidative
stress in patients with ARF caused by compression
injury and Q-wave AMI, as well as the effect of
complemented SCENAR-therapy on this stress. These
researches prove that multiple therapy
complemented with SCENAR-therapy, if compared
with traditional methods. gives better clinical
results by arresting phenomena of oxidative
stress.
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????????? ??????????? ??????-??????? ?? ???????
?????????????? ??????? ? ??????? ? ??? ?
??? This research is aimed at investigating any
specific effects of SCENAR-therapy on the
oxidative stress in patients with ARF and AMI
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?????????? (??), ?????????? ??????????? (???) ?
???????? ????????? (??), ? ????? ??????????
????????? ??????????? ????????????????? ???????
???????? (??) ? ?????????????? (??).
We compared the initial level and dynamics of
primary, secondary and final LOP products in
blood plasma of patients with the indicated
pathology conjugated dienes (CD) malondialdehyde
(MDA) schiff bases (SB) as well as activity of
some components of antioxidative system catalase
(CA) and ceruloplasmin (CP)
19
????????????????? ?????? ???????? ?????????
????????? ? ??????? ???????? ??????-97.4
(??????????????? ????????????? ?? ?? ?
29/23041101/3726-02 ?? 23.05. 2002 ?.).
Patients were treated with SCENAR 97.4
(registration cerificate ? 29/23041101/3726-02
of 05.23.2002)
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I ?????? ???????????? 17 ???????? ? ???,
??????????? ?? ???? ?????????????? ??????.
??????-??????? ????????? ? ???????????-??????????
?? ?????? ??? ??????? ???????????? ? 6 ????? ??
???? ? ???????????? ????, ???, ????????????.
???? ??????? ????????? ? ??????? ???????????
??????? ? ????????? ??????? ??????????? ?????????
? ? ??????? ???????? 8-10 ????????. ????????????
????? ??????????? ?? ?????? ??????? ? ?? 1-2
????? ?????????????? ???????. ???????, ??? ? ????
?????? ??????? ??????? ???? ???????????? ???????
???????? 11,31,3 ???. I group 17 patients
with ARF caused by compression injury. The
patients were treated with SCENAR-therapy using
one of the two general stimulation methods 3
pathways and 6 points and collar zone,
forehead, adrenals in the subjectively dosed
mode (SDM). The treatment course started on
patients admission and included 8-10 daily
sessions. The blood samples for biochemical tests
were taken on admission and on the 1-2 day of
diuresis recovery. It should be noted that
average anuretic period in this group was
11.31.3 days.
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II ?????? ???????????? 30 ???????? ? ??????
Q-?????????? ????????? ????????, ?? ???????????
???????????????? ???????. ??????-??????? ? ????
?????? ????????? ?? ???????? ??? ???????
???????????? ? 6 ????? ?? ????. ?????????????
?????????????? ??????? ?????? ? ??????? 5-10 ???.
? ???? ????? ?????? ????????? (??4, ??6 ? ??7) ??
????? ? ?????? ???? ? ??????? 2-3 ???. ????
??????? ??????? ?? 10-14 ????????, ????????? ?
????????? ? ??????? ??? ???????????. ????????????
????? ??????????? ??? ??????????? ? ?? 16-?? ????
???????????. II group 30 patients with Q-wave
AMI that had contradictions to thrombolytic
therapy. In these group patients were treated
with SCENAR-therapy using one of the two general
stimulation methods 3 pathways and 6 points
and collar zone, forehead, adrenals.
Stimulation of the standard treatment zones was
complemented with stimulation of heart zone (5-10
min) and pericardium meridian points (PC4, PC6
and PC7) on both arms (2-3 minutes). The
treatment course included 10-14 daily sessions
and started at the very first day of the
disease. The blood samples were taken on
admission and on the 16 day of treatment.
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• ??? ??????????? ????????? ??????? ?? ??????????,
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  ??????????? ??????,
• ? ???????? ???????.

In addition to absolute indices of biochemical
parameters under study we will compare their
content (in percent) and conventional norm in the
treatment dynamics
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???????? ?????????? ??, ??? ? ?? ? ?????? ?????
??????? ????? ????? 
Indices of CD, MDA and SB in the blood plasma in
the patients from both groups
? ? ?
? p1gt0,1 ? ?1 pgt0,1 ?1
? ? ?
0,05lt?lt0,1 ?1 0,05lt?lt0,1 ?1 ?gt0,1 0,05lt?1lt0,1
?????????? ? - ????????????? ???????? ?
????????? ? ???????? ?1
- ????????????? ???????? ? ????????? ? ????????
????????? Note p significance of change as
compared with donors p1 significance
of change as compared with initial values
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???????? ?????????? ?? ? ?? ?????? ????? ???????
????? ?????   Indices of CA and CP of the blood
plasma in the patients from both groups
?
p ?1gt0,1 ? 0,05ltp1lt0,1
?gt0,1 0,05lt?1lt0,1 0,05ltPlt0,1 ?1
pgt0,1
0,05lt?lt0,1
?
?????????? ? - ????????????? ???????? ?
????????? ? ???????? ?1 -
????????????? ???????? ? ????????? ? ????????
????????? Note p significance of change as
compared with donors p1 significance
of change as compared with initial values
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????? 1 Conclusion 1

• ? ??????? ? ???????????? ?????????????
  ???????, ? ?????????, ? ?????? ????????
  ????????????????, ??????????? ?? ????
  ?????????????? ??????, ?
• ?????? Q-?????????? ????????? ????????
• ? ?????? ???? ??????????? ??????????? ?????????
• ??????-??????? ?????? ????? ??? ???????
  ?????????????? ???????
• At first days of the disease patients with
  ischemic organ injury, in particular Q-wave
  myocardial infarction and acute renal failure
  caused by compression injury, have oxidative
  stress, which makes the redox balance of the
  blood plasma disordered

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????? 2 Conclusion 2

• ??? ????????? ??????? ??????-??????? ??
  ????????? ??????-??????? ?????? ????? ? ????
  ????????? ??????? ????????
• ????????????? ?????? ?????????? ??????????
  ?????????? ????????? ???, ??? ???????? ?????????
  ?????????????? ?????????? ?????????
  ?????????????.
• ???????????? ?????? ? ????????? ??????? ??
  ????????? ?????????? ??????????? ???????????????
  ??????? ?????? ?????.
• Comparing the effect of multiple treatment
  complemented with SCENAR-therapy on the oxidative
  stress in patients from both groups we detected
• universal effect of inhibiting lipid
  perioxidation high activity of in blood plasma,
  which manifests as decrease in accumulation of
  LOP molecular products
• regulating influence on the state of antioxidant
  system activity of blood plasma

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??????? ?? ????????! Thank you!