Human Genome

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Human Genome

• About 3 billion nucelotide on 23 chromosomes

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OUR AIM

• Integrate all past, present, future info (on
  human genes) in a maintable, user-friendly
  resource.
• 1. Complete human genome (over already)
• 2. Creating a detailed and accurate features
  table of human genome
• 3. Gather these info, oofer them to others

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ENSEMBL

• is a joint project between EBI and Sanger
  Institute
• Aim To offer info about human genome and others
  (mouse)
• A good ccollobration with labs working on human
  genome

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Lets start
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And site map
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• Find where dUTPase is
• Find the location
• Use human
• Go to the aproprate chromosome and site (p or q)
• Select the band

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What some extra

• We can determine the mutaional diseases (becouse
  of SNPs single nucleotide polymorphisime) caused
  SNPs on a specific chromosome.

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• Read your book properly
• Select a different chromosome
• Alpaslan. 1
• Fatih 2
• Bahay3
• Nebahat 4
• etc

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Try other web sites -genome .cse.ucsc.edu
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Protein databases

• One of Most famous ones.. SWISS-PROT, central
  resource on annotated protein sequences cofunded
  by the Swiss Institute of Bioinformatics andEMI
  (Eur. Bioin. Ins.) (derived info. Resource) (by
  Amos)
• Entrez is the next one (repository one)

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A bit about protein

• First thought by bioinformatics ORFs give the
  sequence of protein
• Proteomics analysis data...not exactly true.
• What is the reason
• Modifications on proteins after its translation
  post-translational modifications

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Major role of a protein database

• Display post-trans. Mod. Inf (available after
  experiment or predicted by computers)

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Function of a protein

• Where it goes says what it does
• Zip codes says where it goes
• Possible route
• Cell membrane
• Secreted outside
• Periplasmic membrane
• Mitochondria or others
• Into nucleus

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Structure

• Most proteins have domains
• Each domain can come together in enormous variety
  of forms
• Exp if 2 domain A and B
• Protein can be AA, BB, AB etc
• Recognation and definition of protein domains is
  a major research topic (seen later)

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• Advantages
• Small sequence
• Clear begining and end
• A single strand
• Aminoacids are in the same order

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Parts of a swiis-prot entry

• General info
• Bibliographic info
• Functional info
• Feature table
• Sequence part

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www.expasy.ch/sprot/
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